RT Journal Article SR Electronic(1) A1 Huesgen, Adolfine A1 Huesgen, Gert A1 Stegmann, Johannes A1 Kurth, ReinhardYR 1980 T1 The Immune Response Against the ASV-coded src-Gene Product in Syngeneic Mice JF Journal of General Virology, VO 48 IS 2 SP 401 OP 406 DO https://doi.org/10.1099/0022-1317-48-2-401 PB Microbiology Society, SN 1465-2099, AB SUMMARY The antigenicity of the avian sarcoma virus (ASV)-coded src-gene product pp60src, which is responsible for fibroblast transformation after ASV infection, has been investigated in STU mouse fibrosarcoma cell lines and the corresponding immune response in syngeneic mice has been determined. The development of effective anti-pp60src antibody titres depends on the mode and site of injection of tumour cells and parallels tumour growth. It was found that mouse immunoglobulin heavy chains are unable to serve as substrate for the protein kinase activity of pp60src. Therefore, an indirect protein kinase absorption (PKA) test was initiated to demonstrate recognition of the protein kinase activity associated with the src-gene product. The availability of syngeneic mice and the corresponding ASV-transformed tumour cells should facilitate studies designed to elucidate the possible relationship between the cytoplasmic pp60src and ASV-induced tumour-specific surface antigens (TSSA), for example, by allowing the production of stable mouse hybridomas synthesizing antibodies specific for pp60src and TSSA., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-48-2-401