1887

Abstract

SUMMARY

Seventeen of twenty-six influenza A virus isolates of the H1N1 antigenic subtype and two of eleven H3N2 virus isolates from the 1977–78 season exhibited a phenotype, were restricted in plaquing in MDCK cells at 38.5 °C compared to 34 °C and appeared to be naturally occurring mutants. The cut-off temperature for two such H1N1 virus isolates was established as 38 °C. The viruses were as thermostable as non- isolates and no complementation was detected between the twelve viruses tested. Cloning studies with an H1N1 virus isolate with minimal properties indicated the presence of a mixed population of and particles. Analysis of seven recombinants of A/HK/117/77 (N1N1) virus indicated that the lesion(s) was not located in the NA or HA proteins.

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1980-06-01
2024-04-20
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References

  1. Almond J. W., Mcgeoch D., Barry R. D. 1977; Method for assigning temperature-sensitive mutations of influenza viruses to individual segments of the genome. Virology 81:62–73
    [Google Scholar]
  2. Chanock R. M., Murphy B. R., Spring S. B., Markoff L. J., Richardson L. S., Belshe R. B. 1978; The Use of live mutants for the prevention of respiratory tract disease. Acta Pathologica et Microbiologica Scamiinavica (in the press)
    [Google Scholar]
  3. Ghendon Y. Z., Markushin S. G., Blagovezhenskaya O. V., Genkina D. B. 1975; Study of fowl plaque virus RNA synthesis in temperature-sensitive mutants. Virology 66:454–463
    [Google Scholar]
  4. Hirst G. K. 1973; Mechanism of influenza recombination. I. Factors influencing recombination rates between ts mutants of strain WSN and the classification of mutants into complementation/recombination groups. Virology 55:81–93
    [Google Scholar]
  5. Kendal A. P., Noble G. R., Skehel J. J., Dowdle W. R. 1978; Antigenic similarity of influenza A (HiNi) viruses from epidemics in 1977-78 to ‘Scandinavian’ strains isolated in epidemics of 1950-51. Virology 89:632–636
    [Google Scholar]
  6. Kung H. C., Jen K. F., Yuan W. C., Tien S. F., Chu C. M. 1978; Influenza in China in 1977: recurrence of influenza virus A subtype H1N1. Bulletin of the World Health Organization 56:913–918
    [Google Scholar]
  7. Mackenzie J. S. 1970; Isolation of temperature-sensitive mutants and the construction of a preliminary genetic map for influenza virus. Journal of General Virology 6:63–75
    [Google Scholar]
  8. Murphy B. R., Chanock R. M. 1979; The present status of live influenza A vaccine. Monographs of Paediatrics 11:1–20
    [Google Scholar]
  9. Murphy B. R., Wood F. T., Massicot J. G., Spring S. B., Chanock R. M. 1978; Temperature-sensitive mutants of influenza virus. XV. The genetic and biological characterisation of a recombinant influenza virus containing two ts lesions produced by mating two complementing, single lesion ts mutants. Virology 88:231–243
    [Google Scholar]
  10. Nakajima K., Desselberger U., Palese P. 1978; Recent human influenza A (H1N1) viruses are closely related genetically to strains isolated in 1950. Nature, London 274:334–339
    [Google Scholar]
  11. Palese P., Tobita K., Ueda M., Compans R. W. 1974; Characterisation of temperature sensitive influenza virus mutants defective in neuraminidase. Virology 61:397–410
    [Google Scholar]
  12. Scholtissek C., Kruczinna R., Rott R., Klenk H. D. 1974; Characteristics of an influenza mutant temperature-sensitive for viral RNA synthesis. Virology 58:317–322
    [Google Scholar]
  13. Simpson R. W., Hirst G. K. 1968; Temperature sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation. Virology 35:41–49
    [Google Scholar]
  14. Smorodintsev A. A. 1969; The efficacy of live influenza vaccines. Bulletin of the World Health Organization 41:585–588
    [Google Scholar]
  15. Spring S. B., Nusinoff S. R., Tierney E. L., Richman D. D., Murphy B. R., Chanock R. M. 1975; Teitl- perature-sensitive mutants of influenza. VIII. Genetic and biological characterization of ts mutants of influenza virus A (H3N2) and their assignment to complementation groups. Virology 66:542–550
    [Google Scholar]
  16. Sugiura A., Tobita K., Kilbourne E. D. 1972; Isolation and preliminary characterization of temperature- sensitive mutants of influenza virus. Journal of Virology 10:639–647
    [Google Scholar]
  17. Ueda M., Kilbourne E. D. 1976; Temperature sensitive mutants of influenza virus: a mutation in the haemagglutinin gene. Virology 70:425–431
    [Google Scholar]
  18. Webster R. G., Kendal A. P., Gerhard W. 1979; Analysis of antigenic drift in recently isolated influenza A (HiNi) viruses using monoclonal antibody preparations. Virology 96:258–264
    [Google Scholar]
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