1887

Abstract

SUMMARY

A simple and highly sensitive radioimmunosorbent assay (RISA) for the detection of plant viruses is described. The RISA procedure is a microplate method based on the principle of ‘double-antibody sandwich’ and follows essentially the protocol of the enzyme-linked immunosorbent assay (ELISA) (Clark & Adams, 1977), with the exception that I-labelled -globulin is substituted for the -globulin enzyme conjugate; the bound I--globulin is dissociated by acidification from the double-antibody sandwich. The radioactivity is proportional to virus concentration, and cauliflower mosaic virus (CaMV) and lettuce mosaic virus (LMV) could be detected at concentrations as low as 5 and 2 ng/ml, respectively. Direct evidence of the adverse effects of conjugation with enzyme on the binding abilities of antibodies is presented. The RISA procedure should prove valuable with viruses for which the ELISA values are too low to be dependable.

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/content/journal/jgv/10.1099/0022-1317-48-2-311
1980-06-01
2024-04-25
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References

  1. Ball E. M. 1973; Solid phase radioimmunoassay for plant viruses. Virology 55:516–520
    [Google Scholar]
  2. Beier H., Shepherd R. J. 1978; Serologically specific electron microscopy in the quantitative measurement of two isometric viruses. Phytopathology 68:533–538
    [Google Scholar]
  3. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme–linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  4. Converse R. H. 1978; Detection of tomato ringspot virus in red raspberry by enzyme-linked immunosorbent assay (ELISA). Plant Disease Reporter 62:189–192
    [Google Scholar]
  5. Daugharty H., Ziegler D. W. 1977; Radioimmunoassay in viral diagnosis. In Comparative Diagnosis of Viral Diseases pp 459–487 Edited by Kurstak E., Kurstak C. New York: Academic Press;
    [Google Scholar]
  6. Fraker P. J., Speck J. C. Jun 1978; Protein and cell membrane iodinations with a sparingly soluble chloramide, l, 3, 4, 6–tetrachloro–3tf, 6a-diphenyIglycoluril. Biochemical and Biophysical Research Communications 80:849–857
    [Google Scholar]
  7. Gera A., Lobenstein G., Raccah B. 1978; Detection of cucumber mosaic virus m viruliferous aphids by enzyme-linked immunosorbent assay. Virology 86:542–545
    [Google Scholar]
  8. Hamilton R. I., Nichols C. 1978; Serological methods for detection of pea seed-borne mosaic virus in leaves and seeds of Pisum sativum. Phytopathology 68:539–543
    [Google Scholar]
  9. Hull R., Shepherd R. J., Harvey J. D. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles. Journal of General Virology 31:93–100
    [Google Scholar]
  10. Jafarpour B., Shepherd R. J., Grogan R. G. 1979; Serological detection of bean common mosaic and lettuce mosaic viruses in seed. Phytopathology 69:1125–1129
    [Google Scholar]
  11. Koenig R. 1978; ELISA in the study of homologous and heterologous reactions of plant viruses. Journal of General Virology 40:309–318
    [Google Scholar]
  12. Koenig R., Dalchow J., Lesemann D. E., Preil W. 1979; ELISA as a reliable tool for the detection of chrysanthemum virus B in chrysanthemum throughout the year. Plant Disease Reporter 69:301–303
    [Google Scholar]
  13. Lister R. M. 1978; Application of the enzyme-linked immunosorbent assay for detecting viruses in soybean seed and plants. Phytopathology 68:1393–1400
    [Google Scholar]
  14. Ramsdell D. C., Andrews R. W., Gillett J. M., Morris C. E. 1979; A comparison between enzyme-linked immunosorbent assay (ELISA) and Chenopodium quinoa for detection of peach rosette mosaic virus in ‘Concord’ grapevines. Plant Disease Reporter 63:74–78
    [Google Scholar]
  15. Reeves J. T., Jackson A. O., Paschke J. D., Lister R. M. 1978; Use of enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of two maize viruses. Plant Disease Reporter 62:667–671
    [Google Scholar]
  16. Shepherd R. J. 1976; DNA viruses of higher plants. Advances in Virus Research 20:305–339
    [Google Scholar]
  17. Veerisetty V., Brakke M. K. 1978; Purification of some legume Carlaviruses. Phytopathology 68:59–64
    [Google Scholar]
  18. Voller A., Bartlett A., Bidwell D. R., Clark M. F., Adams A. N. 1976; The detection Of viruses by enzyme-linked immunosorbent assay (ELISA). Journal of General Virology 33:165–167
    [Google Scholar]
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