RT Journal Article SR Electronic(1) A1 Neurath, A. R. A1 Stevens, C. E. A1 Strick, N. A1 Szmuness, W. A1 Oleszko, W. R. A1 Harley, E. J.YR 1980 T1 An Antigen Detected Frequently in Human Sera With Elevated Levels of Alanine Aminotransferase: A Potential Marker For Non-A, Non-B Hepatitis JF Journal of General Virology, VO 48 IS 2 SP 285 OP 295 DO https://doi.org/10.1099/0022-1317-48-2-285 PB Microbiology Society, SN 1465-2099, AB SUMMARY In a search for additional antigens associated with virus-induced human liver disease a radioimmunoassay (RIA) was developed using IgG from sera of a multiply transfused person. Polystyrene beads coated with IgG F(ab)′2 fragments, dinitrophenylated F(ab)′2 fragments and 125I-labelled anti-2,4-dinitrophenyl antibodies (Neurath & Strick, 1979) were used in the RIA. An apparently new antigen or the corresponding antibodies were detected in 155 serum specimens from 35/37 (94%) individuals who developed non-A, non-B hepatitis. The antigen was also present in hepatitis B surface antigen-negative sera of blood donors with normal (13.2%) and elevated levels of alanine aminotransferase (34%). The antigen has an approximate mol. wt. of 45000, a buoyant density of 1.23 g/ml and an isoelectric point of 7., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-48-2-285