1887

Abstract

SUMMARY

Four major polypeptides with mol. wt. of 22000, 25000, 52000 and 68000 were isolated from solubilized preparations of hepatitis type B surface antigen (HBsAg). These four populations, referred to as P22, P25, P52 and P68, respectively, were used to immunize guinea-pigs. Guinea-pigs were also inoculated with HBsAg and with purified human serum albumin (HuSA). These antisera were utilized to establish that intact HBsAg particles are associated with HuSA antigenic reactivity. HuSA antigenic determinants were associated with purified preparations of P68. HuSA antigenic activity was not detected with purified preparations of P22, P25 and P52 or with the respective specific antisera to each of the above. However, purified P68 contained the antigenic determinants of both host protein and hepatitis B virus-specified protein origin.

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1980-06-01
2024-03-28
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References

  1. Alexander J., Macnab G., Saunders R. 1978; Studies on in vitro production of hepatitis B surface antigen by a human hepatoma cell line. In Perspectives in Virology vol 10 pp 103–120 Edited by Pollard M. New York: Raven Press;
    [Google Scholar]
  2. Almeida J. D. 1972; Individual morphological variations seen in Australia antigen positive sera. American Journal of Diseases of Children 123:303–309
    [Google Scholar]
  3. Avrameas S., Ternynck T. 1969; The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry 6:53–66
    [Google Scholar]
  4. Burrell C. J. 1975; Host components in hepatitis B antigens. Journal of General Virology 27:117–126
    [Google Scholar]
  5. Cabral G. A., Marciano-Cabral F., Funk G. A., Sanchez Y., Hollinger F. B., Melnick J. L., Dreesman G. R. 1978; Cellular and humoral immunity in guinea pigs to two major polypeptides derived from hepatitis B surface antigen. Journal of General Virology 38:339–350
    [Google Scholar]
  6. Carlsson H. E., Perlmann P. 1969; Isoelectric focusing and immunological characterization of human serum albumin. In Protides of the Biological Fluids vol 17 section C: Electrofocusing pp 439–442 Edited by Peeters H. New York: Pergamon Press;
    [Google Scholar]
  7. Chairez R., Hollinger F. B., Melnick J. L., Dreesman G. R. 1974; Biophysical properties of purified forms of hepatitis B antigen. Jntervirology 3:129–140
    [Google Scholar]
  8. Chairez R., Hollinger F. B., Brunschwig J. P., Dreesman G. R. 1975; Comparative biophysical studies of hepatitis B antigen, subtypes adw and ayw. Journal of Virology 15:182–190
    [Google Scholar]
  9. Chisari F. V., Gerin J. L., Edgington T. S. 1974; Immunochemistry of the hepatitis B virus: 125I-HB Ag ligand. Journal of Immunology 113:543–553
    [Google Scholar]
  10. Dreesman G. R., Gerin J. L. 1978; Discussion: structure of hepatitis viruses. In Viral Hepatitis pp 645–648 Edited by Vyas G. N., Cohen S. N., Schmid R. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  11. Dreesman G. R., Hollinger F. B., Mccombs R. M., Melnick J. L. 1972a; Production of potent anti-Australia antigen sera of high specificity and sensitivity in goats. Infection and Immunity 5:213–221
    [Google Scholar]
  12. Dreesman G. R., Hollinger F. B., Suriano J. R., Fujioka R. S., Brunschwig J. P., Melnick J. L. 1972b; Biophysical and biochemical heterogeneity of purified hepatitis B antigen. Journal of Virology 10:469–476
    [Google Scholar]
  13. Gerin J. L. 1974; Structure of hepatitis B antigen (HBAg). In Mechanisms of Virus Disease pp 215–223 Edited by Robinson W. S., Fox C. F. Menlo Park, Calif.: W. A. Benjamin;
    [Google Scholar]
  14. Gerlich W., May G. 1973; Hepatitis-associated antigen: purification and properties. Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, I. Abteilung Originate, Reihe A 224:49–60
    [Google Scholar]
  15. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of mI-labeled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  16. Hollinger F. B. 1974; Hepatitis viruses. In Manual of Clinical Microbiology 2nd edn pp 819–833 Edited by Lennette H. E., Spaulding E. H., Truant J. P. Washington, D,. C.: American Society for Microbiology;
    [Google Scholar]
  17. Hollinger F. B., Vorndam V., Dreesman G. R. 1971; Assay of Australia antigen and antibody employing double-antibody and solid-phase radioimmunoassay techniques and comparison with passive hemagglutination methods. Journal of Immunology 107:1099–1111
    [Google Scholar]
  18. Hollinger F. B., Morrison M., Chairez R., Dreesman G. R. 1975; Immunological and biophysical properties of hepatitis B antigen labeled by the chloramine-T and by the lactoperoxidase methods. Journal of Immunological Methods 8:67–84
    [Google Scholar]
  19. Imai M., Yanase Y., Nojiri T., Miyakawa Y., Mayumi M. 1979; A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology 76:242–247
    [Google Scholar]
  20. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  21. Millman I., Hutanen H., Merino F., Bayer M. E., Blumberg B. S. 1971; Australia antigen: physical and chemical properties. Research Communications in Chemical Pathology and Pharmacology 2:667–686
    [Google Scholar]
  22. Monjardino J., Crawford E. 1979; Polypeptide profile of HBsAg excreted by a human hepatoma cell line. Virology 96:652–655
    [Google Scholar]
  23. Neurath A. R., Strick N. 1979; Radioimmunoassay for albumin-binding sites associated with the presence of e-antigen in serum. Intervirology 11:128–132
    [Google Scholar]
  24. Neurath A. R., Prince A. M., Lippin A. 1974; Antigenic sites related to human serum proteins revealed by affinity chromatography. Proceedings of the National Academy of Sciences of the United States of America 71:2663–2667
    [Google Scholar]
  25. Richards F. F., Konigsberg W. H., Rosenstein R. W., Varga J. M. 1975; On the specificity of antibodies. Science 187:130–137
    [Google Scholar]
  26. Robinson W. S. 1977; The genome of hepatitis B virus. Annual Review of Microbiology 31:357–377
    [Google Scholar]
  27. Secher D. S. 1977; Multispecific antibodies. Nature, London 268:689–690
    [Google Scholar]
  28. Skelly J., Howard C. R., Zuckerman A. J. 1979; Analysis of hepatitis B surface antigen components solubilized with Triton X-100. Journal of General Virology 44:679–689
    [Google Scholar]
  29. Spira G., Biswal N., Dreesman G. R. 1974; A modified radioimmunoassay for murine sarcoma-leukemia virus group-specific antigen. Applied Microbiology 28:239–244
    [Google Scholar]
  30. Stannard L. M., Moodie J. W. 1976; Hepatitis B antigen: IgG components shown by immune electron microscopy. Journal of General Virology 30:269–272
    [Google Scholar]
  31. Vnek J., Prince A. M., Hashimoto W., Ikram H. 1978; Association of normal serum protein antigens with chimpanzee hepatitis B surface antigen particles. Journal of Medical Virology 2:319–333
    [Google Scholar]
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