1887

Abstract

SUMMARY

We have shown that a single defective-interfering (DI) particle of early (5th) passage Sindbis virus induces maximal amounts of interferon in an ‘aged’ primary chick embryo cell. The capacity of such DI particles to induce interferon is inactivated by small amounts of u.v. radiation (1/e dose = 232 ergs/mm). The 1/e dose for inactivation of the interferon-inducing capacity of infectious virus particles is 399 ergs/mm and for infectivity is 1010 ergs/mm. Pre-treatment with interferon blocks formation of interferon in response to either DI or infectious virus particles. Our results suggest that Sindbis virus genes must be expressed to form the interferon inducer, which is presumably a molecule of double-stranded (ds)RNA. We postulate that for interferon induction, the genomic RNA which codes for genes G and A must be translated into products whose concerted action produces a dsRNA molecule upon synthesis of a segment of RNA complementary to the genome. The RNA from early passage DI particles is sufficiently large (25S, 1.6 × 10 mol. wt.) to accommodate these genes, whereas the RNA from the late passage DI particles (20S, 1.0 × 10 mol. wt.) is not. Late (15th) passage DI particles do not induce interferon formation.

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1980-05-01
2021-10-27
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References

  1. Bellett A. J. D., Cooper P. D. 1959; Some properties of the transmissible interfering component of vesicular stomatitis virus preparations. Journal of General Microbiology 21:498–509
    [Google Scholar]
  2. Bruton C. J., Kennedy S. I. T. 1976; Defective-interfering particles of Semliki Forest virus: structural differences between standard virus and defective-interfering particles. Journal of General Virology 31:383–395
    [Google Scholar]
  3. Bruton C. J., Porter A., Kennedy S. I. T. 1976; Defective-interfering particles of Semliki Forest virus: intracellular events during interference. Journal of General Virology 31:397–416
    [Google Scholar]
  4. Brzeski H., Kennedy S. I. T. 1978; Synthesis of alphavirus-specified RNA. Journal of Virology 25:630–640
    [Google Scholar]
  5. Carver D. H., Marcus P. I. 1967; Enhanced interferon production from chick embryo cells aged in vitro. Virology 32:247–257
    [Google Scholar]
  6. Carver D. H., Marcus P. I. 1968; Rubella virus as an arbovirus. Bacteriological Proceedings p 181
    [Google Scholar]
  7. Clewley J. P. C., Kennedy S. I. T. 1976; Purification and polypeptide composition of Semliki forest virus RNA polymerase. Journal of General Virology 32:395–411
    [Google Scholar]
  8. Collins P. L., Hightower L. E., Ball L. A. 1978; Transcription and translation of Newcastle disease virus raRNA′s in vitro. Journal of Virology 28:324–336
    [Google Scholar]
  9. Fuller F. J., Marcus P. I. 1979; Cell surface-associated ribonuclease activities. Journal of Cellular Physiology 98:1–10
    [Google Scholar]
  10. Fuller F. J., Marcus P. I. 1980; Interferon induction by viruses. V. Sindbis virus: defective-interfering particles temperature-sensitive for interferon induction. Journal of General Virology 48: (in the press)
    [Google Scholar]
  11. Guild G. M., Stollar V. 1975; Defective-interfering particles of Sindbis virus. III. Intracellular viral RNA species in chick embryo cell cultures. Virology 67:24–41
    [Google Scholar]
  12. Guild G. M., Stollar V. 1977; Defective-interfering particles of Sindbis virus. V. Sequence relationships between SVSTD RNA and intracellular defective viral RNAs. Virology 77:175–188
    [Google Scholar]
  13. Heller E. 1963; Enhancement of Chikungunya virus replication and inhibition of interferon production by actinomycin D. Virology 21:652–656
    [Google Scholar]
  14. Johnston R. E., Tovells D. R., Brown D. T., Faulkner P. 1975; Interfering passages of Sindbis virus: concomitant appearance of interference, morphological variants and truncated viral RNA. Journal of Virology 16:951–958
    [Google Scholar]
  15. Kennedy S. I. T., Bruton C. J., Weiss B., Schlesinger S. 1976; Defective interfering passages of Sindbis virus: nature of the defective virion RNA. Journal of Virology 19:1034–1043
    [Google Scholar]
  16. Kowal K., Flores L., Stollar V. 1979; U,. V. irradiation of standard and DI particles of Sindbis virus. American Society for Microbiology (Abstracts) p 258
    [Google Scholar]
  17. Lazzarini R. A., Weber G. H., Johnston L. D., Stamminger G. M. 1975; Covalently linked message and anti-message (genomic) RNA from a defective vesicular stomatitis virus particle. Journal of Molecular Biology 97:289–307
    [Google Scholar]
  18. Marcus P. I. 1959; Single cell techniques in tracing virus-host interactions. Bacteriological Reviews 23:232–249
    [Google Scholar]
  19. Marcus P. I., Fuller F. J. 1979; Interferon induction by viruses. II. Sindbis virus: interferon induction requires one-quarter of the genome -genes G and A. Journal of General Virology 44:169–177
    [Google Scholar]
  20. Marcus P. T., Sekeluck M. J. 1974; Cell killing by viruses. I. Comparison of cell-killing plaque-forming and defective-interfering particles of vesicular stomatitis virus. Virology 57:321–338
    [Google Scholar]
  21. Marcus P. I., Sekellick M. J. 1977; Defective-interfering particles with covalently linked [±] RNA induce interferon. Nature, London 266:815–819
    [Google Scholar]
  22. MarcuS P. I., SekellicK M. J. 1980; Interferon induction by viruses. III. Vesicular stomatitis virus: interferon-inducing particle activity requires partial transcription of gene N. Journal of General Virology 47:89–96
    [Google Scholar]
  23. Schlesinger S., Schlesinger M., Burge B. W. 1972; Defective virus particles from Sindbis virus. Virology 47:615–617
    [Google Scholar]
  24. Sekellick M. J., Marcus P. I. 1978; Persistent infection. I. Interferon-inducing defective-interfering particles as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 85:175–186
    [Google Scholar]
  25. Sekellick M. J., Marcus P. T. 1979; Persistent infections of rhabdoviruses. In Rhabdoviruses (in the press) Edited by Bishop D. H. L. Florida, U.S.A.: CRC Press;
    [Google Scholar]
  26. Shenk T. E., Stollar V. 1972; Viral RNA species in BHK-21 cells infected with Sindbis vims serially passaged at high multiplicity of infection. Biochemical and Biophysical Research Communications 49:60–67
    [Google Scholar]
  27. Simmons D. T., Strauss J. H. 1972; Replication of Sindbis virus. I. Relative size and genetic content of 26S and 49S RNA. Journal of Molecular Biology 71:599–613
    [Google Scholar]
  28. Stark C., Kennedy S. I. T. 1978; The generation and propagation of defective-interfering particles of Semliki forest virus in different cell types. Virology 89:285–299
    [Google Scholar]
  29. Stewart W. E. II 1979; Priming. In The Interferon System pp 233–236 Wien, N,. Y.: Springer-Verlag;
    [Google Scholar]
  30. Stollar V. 1979; Defective-interfering particles of togaviruses. Current Topics in Microbiology & Immunology (in the press)
    [Google Scholar]
  31. Vilček J., Rada B. 1962; Studies on an interferon from tick-borne encephalitis virus infected cells. III. Antiviral action of interferon. Acta Virologica 6:9–15
    [Google Scholar]
  32. Weiss B., Schlesinger S. 1973; Defective-interfering passages of Sindbis virus: chemical composition, biological activity and mode of interference. Journal of Virology 12:862–871
    [Google Scholar]
  33. Weiss B., Goran D., Cancedda R., Schlesinger S. 1974; Defective-interfering passages of Sindbis virus: nature of the intracellular defective viral RNA. Journal of Virology 14:1189–1198
    [Google Scholar]
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