1887

Abstract

SUMMARY

DNA replication complexes were purified from adenovirus type 5 (Ad5)-infected HeLa cells. DNA synthesis by these complexes was extremely sensitive to the inhibitors dideoxythymidine triphosphate, -ethyl maleimide and -hydroxymercuribenzoate. The bound DNA polymerase was released from the complexes by limited digestion with micrococcal nuclease. This released polymerase preferred poly(rA):(dT) as template over activated calf thymus DNA. These results are compatible with the major polymerase in the replication complex being of the class.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-48-1-231
1980-05-01
2022-10-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/48/1/JV0480010231.html?itemId=/content/journal/jgv/10.1099/0022-1317-48-1-231&mimeType=html&fmt=ahah

References

  1. Abboud M. M., Horwitz M. S. 1979; The DNA polymerase associated with the adenovirus type 2 replication complex: effect of 2′3 dideoxythymidine 5′ triphosphate on viral DNA synthesis. Nucleic Acids Research 6:1025–1039
    [Google Scholar]
  2. Arens M., Yamashita T., Padmanabhan R., Tsuruo T., Green M. 1977; Adenovirus deoxyribonucleic acid replication. Characterization of the enzyme activities of a soluble replication complex. Journal of Biological Chemistry 252:7947–7954
    [Google Scholar]
  3. Bolden A., Pedrali Noy O., Weissbach A. 1977; DNA polymerase of mitochondria is y polymerase. Journal of Biological Chemistry 252:3351–3356
    [Google Scholar]
  4. Borst P., Grivell L. A. 1978; The mitochondrial genome of yeast. Cell 15:705–724
    [Google Scholar]
  5. Brison O., Kedinger C., Wilhelm J. 1977; Enzymatic properties of viral replication complexes isolated from adenovirus type 2 infected HeLa cell nuclei. Journal of Virology 24:423–435
    [Google Scholar]
  6. Edenberg H. J., Anderson S., Depamphilis M. L. 1978; Involvement of DNA polymerase a. in simian virus 40 DNA replication. Journal of Biological Chemistry 253:3273–3280
    [Google Scholar]
  7. Fisher P. B., Horwitz M. S. 1977; Mitochondrial DNA synthesis in adenovirus type 2 infected HeLa cells. Journal of Virology 22:340–345
    [Google Scholar]
  8. Frenkel G. D. 1978; Adenovirus DNA synthesis in vitro in an isolated complex. Journal of Virology 25:459–463
    [Google Scholar]
  9. De K., Arens M., Green M. 1976; Characterization of DNA polymerase associated with nuclear membrane fractions from uninfected and adenovirus 2 infected KB cells. Biochimica et Biophysica Acta 447:340–352
    [Google Scholar]
  10. De Jong A., Van Der Vliet P. C., Jansz H. S. 1977; DNA polymerases in adenovirus type 5 infected and uninfected KB cells. Induction of an a type DNA polymerase in adenovirus type 5 infected fast growing cells. Biochimica et Biophysica Acta 476:156–165
    [Google Scholar]
  11. Longiaru M., Ikeda J.-E., Jarkovsky Z., Horwitz S. B., Horwitz M. S. 1979; The effect of aphidicolin on adenovirus DNA synthesis. Nucleic Acids Research 6:3369–3386
    [Google Scholar]
  12. Mantyjarvi R., Russell W. C. 1969; Studies on DNA synthesis in cells infected with adenovirus. Journal of General Virology 5:339–350
    [Google Scholar]
  13. Matsukage A., Bohn E. W., Wilson S. H. 1975; Differential sensitivity of low molecular weight DNA polymerase to sulphydryl blocking agents. Biochimica et Biophysica Acta 383:338–343
    [Google Scholar]
  14. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  15. Robberson D. L., Kasamatsu M., Vinograd J. 1972; Replication of mitochondrial DNA. Circular replication intermediates in mouse L cells. Proceedings of the National Academy of Sciences of the United States of America 69:737–741
    [Google Scholar]
  16. Shaw C. H. 1979; A study of adenovirus DNA replication. Ph.D. thesis CNAA, London:
    [Google Scholar]
  17. Shaw C. H., Rekosh D. M., Russell W. C., Blair G. E. 1978; Adenovirus DNA synthesis in a soluble fraction isolated from infected human cells. Biochemical Society Transactions 6:752–753
    [Google Scholar]
  18. Shaw C. H., Russell W. C., Rekosh D. M. 1979; Association of adenovirus early proteins with a nuclear fraction that synthesises DNA in vitro. Virology 92:436–448
    [Google Scholar]
  19. Van der Vliet P. C., Kwant M. M. 1978; Role of DNA polymerase y in adenovirus DNA replication. Nature, London 276:532–534
    [Google Scholar]
  20. Wang T. S.-F., Sedwick D., Korn D. 1974; Nuclear deoxyribonucleic acid polymerase. Purification and properties of the homogeneous enzyme from human KB cells. Journal of Biological Chemistry 249:841–850
    [Google Scholar]
  21. Waqar M. A., Evans M. J., Huberman J. A. 1978; Effect of 2′3′ dideoxythymidine 5′ triphosphate on HeLa cells in vitro DNA synthesis: evidence that DNA polymerase a is the only polymerase required for cellular DNA replication. Nucleic Acids Research 5:1933–1946
    [Google Scholar]
  22. Weissbach A. 1977; Eukaryotic DNA polymerases. Annual Review of Biochemistry 46:25–47
    [Google Scholar]
  23. Weissbach A., Schlabach A., Fridlender B., Bolden A. 1971; DNA polymerases from human cells. Nature New Biology 231:167–170
    [Google Scholar]
  24. Wilhelm J., Brison O., Kedinger C., Chambon P. 1976; Characterization of adenovirus type 2 transcriptional complexes isolated from infected HeLa cell nuclei. Journal of Virology 19:61–81
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-48-1-231
Loading
/content/journal/jgv/10.1099/0022-1317-48-1-231
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error