1887

Abstract

Summary

DNA replication complexes were purified from adenovirus type 5 (Ad5)-infected HeLa cells. DNA synthesis by these complexes was extremely sensitive to the inhibitors dideoxythymidine triphosphate, -ethyl maleimide and -hydroxymercuribenzoate. The bound DNA polymerase was released from the complexes by limited digestion with micrococcal nuclease. This released polymerase preferred poly(rA):(dT) as template over activated calf thymus DNA. These results are compatible with the major polymerase in the replication complex being of the γ class.

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/content/journal/jgv/10.1099/0022-1317-48-1-231
1980-05-01
2019-11-20
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-48-1-231
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