Indirect Enzyme-linked Immunosorbent Assay (ELISA) for the Detection of Coxsackievirus Group B Antibodies Free

Abstract

SUMMARY

An indirect, solid phase, microplate enzyme-linked immunosorbent assay (ELISA) was found to be highly sensitive and reliable for detecting antibodies to the group B Coxsackieviruses and other picornaviruses. Conditions for obtaining maximum sensitivity and reproducibility of the indirect ELISA are described. Antibody titres were comparable to those obtained by the virus neutralization test and over 50 times higher than those obtained by the complement-fixation test. Purified virions used in the indirect ELISA reacted with low levels of cross-reacting heterotypic antibodies elicited by each of the six group B Coxsackieviruses, although homotypic reactions resulted in highest titres.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-48-1-225
1980-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/48/1/JV0480010225.html?itemId=/content/journal/jgv/10.1099/0022-1317-48-1-225&mimeType=html&fmt=ahah

References

  1. Crowell R. L. 1963; Specific viral interference in HeLa cell cultures chronically infected with Coxsackie B5 virus. Journal of Bacteriology 86:517–526
    [Google Scholar]
  2. Crowell R. L., Philipson L. 1971; Specific alterations of Coxsackievirus B3 eluted from HeLa cells. Journal of Virology 8:509–515
    [Google Scholar]
  3. Crowell R. L., Syverton J. T. 1961; The mammalian cell virus relationship. VI. Sustained infection of HeLa cells by Coxsackie B3 virus and effect on superinfection. Journal of Experimental Medicine 113:419–435
    [Google Scholar]
  4. Engvall E., Perlmann P. 1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  5. Gilman S. C., Docherty J. J. 1977; Detection of antibodies specific for herpes simplex virus in human sera by the enzyme-linked immunsorbent assay. Journal of Infectious Diseases, Supplement 136:S286–S293
    [Google Scholar]
  6. Gravell M., Dorsett P., Guttenson O., Ley A. C. 1977; Detection of antibody to rubella virus by enzyme-linked immunosorbent assay. Journal of Infectious Diseases, Supplement 136:S300–S303
    [Google Scholar]
  7. Kraft L. M., Melnick J. L. 1950; Immunological reactions of the Coxsackieviruses. II. The complement fixation test. Journal of Experimental Medicine 92:483–497
    [Google Scholar]
  8. Lennette E. H., Melnick J. L., Magoffin R. L. 1974; Clinical virology: introduction to method. In Manual of Clinical Microbiology pp 667–677 Edited by Lennette E. H., Spaulding E. H., Truant J. P. Washington, D,. C.: American Society of Microbiology;
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Macwilliam K. M., Cooper M. A. 1974; Antibody levels in human sera measured by the fluorescent-antibody technique against the Coxsackie B viruses types 1 to 5 grown in Hep 2 cells compared with results obtained by neutralization. Journal of Clinical Pathology 27:825–827
    [Google Scholar]
  11. Melnick J. L., Ledinko N. 1950; Immunological reactions of the Coxsackievirus. I. The neutralization test: techniques and applications. Journal of Experimental Medicine 92:463–482
    [Google Scholar]
  12. Schmidt N. J., Dennis J., Frommhagen L. H., Lennette E. H. 1963; Serologic reactivity of certain antigens obtained by fractionation of Coxsackie viruses in cesium chloride density gradients. Journal of Immunology 90:654–662
    [Google Scholar]
  13. Schmidt N. J., Lennette E. H. 1962; Gel double diffusion studies with group B and group A, type 9 coxsackie viruses. I. The technique and reactions obtained with hyperimmune animal sera and human sera. Journal of Immunology 89:85–95
    [Google Scholar]
  14. Svehag S. E., Mandel B. 1964; The formation and properties of poliovirus-neutralizing antibody. I. 19S and 7S antibody formation: difference in kinetics and antigen dose requirements for induction. Journal of Experimental Medicine 119:1–19
    [Google Scholar]
  15. Voller A., Bidwell D. E. 1976; Enzyme-immunoassays for antibodies in measles, cytomegalovirus infection and after rubella vaccination. British Journal of Experimental Pathology 57:243–247
    [Google Scholar]
  16. Voller A., Bidwell D., Huldt G., Engvall E. 1974; A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bulletin of the World Health Organization 51:209–211
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-48-1-225
Loading
/content/journal/jgv/10.1099/0022-1317-48-1-225
Loading

Data & Media loading...

Most cited Most Cited RSS feed