@article{mbs:/content/journal/jgv/10.1099/0022-1317-48-1-213, author = "Collins, M. S. and Alexander, D. J.", title = "The Polypeptide Composition of Isolated Surface Projections of Avian Infectious Bronchitis Virus", journal= "Journal of General Virology", year = "1980", volume = "48", number = "1", pages = "213-217", doi = "https://doi.org/10.1099/0022-1317-48-1-213", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-48-1-213", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 m-KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 m-KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86000 and 66000 mol. wt. The larger polypeptide was glycosylated.", }