1887

Abstract

Summary

Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 -KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 -KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86000 and 66000 mol. wt. The larger polypeptide was glycosylated.

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/content/journal/jgv/10.1099/0022-1317-48-1-213
1980-05-01
2019-10-17
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-48-1-213
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