The DNA sequence homology relationships among six lepidopteran nuclear polyhedrosis viruses (NPVs) have been explored by hybridization of P-labelled NPV DNAs to Southern blots of restriction endonuclease-digested NPV DNA. The NPV (AcMNPV) shows extensive DNA sequence homology throughout the entire genome with the NPV (RoMNPV). Both the NPV (OpMNPV) and the NPV (PdMNPV) share homologous regions, equivalent to 1% of the DNA genome, with AcMNPV and RoMNPV. This homology is localized in two regions on the AcMNPV physical map although other regions are also weakly homologous. Approx. 1% of the DNA of OpMNPV and PdMNPV show sequence homology with each other; the homology is primarily localized in two to four regions of the genomes. NPV (HzSNPV) and NPV (TnSNPV) share less than 0.2% sequence homology with the MNPVs and share less than 0.2% sequence homology with each other.


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