The polypeptides induced by canine distemper virus (CDV) strains have been characterized by polyacrylamide slab gel electrophoresis of infected cell lysates labelled with S-methionine, C-amino acids, H-glucosamine and H-mannose, or P-orthophosphate. Seven virus-induced polypeptides have been assigned the following nomenclature and mol. wt.: a large polypeptide L (180000); a large glycoprotein H (77000); a nucleocapsid-associated protein P (73000); the nucleocapsid protein N (60000); the smaller glycoprotein F (59000); a membrane protein M (35000) and a small polypeptide S (15000). During pulse-chase experiments with H-glucosamine and C-amino acids the intensity of the F band decreases and that of the F and F bands increases; the H polypeptide band becomes more diffuse and the S-protein disappears. The N- and P- but not the M-proteins have been found to be phosphorylated. The polypeptide pattern of the Onderstepoort strain of CDV has been compared with that of two other CDV and with 17 measles and subacute sclerosing panencephalitis (SSPE) strains. Differences in the mobilities of various polypeptides have been observed between CDV and measles and SSPE strains; however, the only consistent difference is the mol. wt. of the M-protein of CDV strains which is smaller by 2000 than that of MV and this may be a biochemical marker to distinguish CDV from measles and SSPE virus strains.


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