1887

Abstract

SUMMARY

Canine thymus cells infected with virus (HL-23V) produced by human acute myelogenous leukaemia cells in culture were shown in previous reports to produce transforming and non-transforming type C virus similar or identical to the simian sarcoma virus complex SSV(SSAV) and to induce tumours in marmoset monkeys (Bergholz ., 1977). In these earlier studies the appearance of breakthrough foci at low dilutions of antiserum in neutralization tests with high-titred anti-SSV(SSAV) serum suggested the presence of another virus, distinct from SSV-(SSAV). We now report the isolation of this component and, by comparative neutralization analysis, demonstrate that it is most closely related to gibbon ape leukaemia virus (GALV). It is distinguished from SSV(SSAV) by kinetics of neutralization and molecular hybridization experiments. This component was readily cloned both from virus produced by HL-23V chronically-infected canine thymus cells established by Teich ., (1975) when HL-23V was first isolated and from virus produced by HL-23V-induced marmoset tumour cells in culture. The presence of this component in the original leukaemic cell cultures is discussed.

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1980-05-01
2022-01-26
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References

  1. Benveniste R., Todaro O. J. 1973; Homology between type C viruses of various species as determined by molecular hybridization. Proceedings of the National Academy of Sciences of the United States of America 70:3316–3320
    [Google Scholar]
  2. Benveniste R., Todaro O. J. 1978; Evolution of primate oncornaviruses: an endogenous virus from langurs (Presbytis spp.) with related virogene sequences in other Old World monkeys. Proceedings of the National Academy of Sciences of the United States of America 74:4557–4561
    [Google Scholar]
  3. Bergholz C. M., Wolff L. G., Deinhardt F., Thakkar B., Marczynka B. 1977; Oncogenicity in marmosets of HL-23V, a type C oncornavirus isolated from human leukemia cells and comparison with simian sarcoma virus type 1 (SSV-1/SSAV-1). Journal of the National Cancer Institute 58:1041–1046
    [Google Scholar]
  4. Bergholz C. M., Wolfe L. G., Deinhardt F. 1977b; Establishment of simian sarcoma virus type 1 (SSV-1)-transformed nonproducer marmoset cell lines. International Journal of Cancer 20:104–111
    [Google Scholar]
  5. Birnsteil M. L., Sells B. H., Purdom L. F. 1972; Kinetic complexity of RNA molecules. Journal of Molecular Biology 63:21–35
    [Google Scholar]
  6. Chan E., Peters W. P., Sweet R. W., Ohno T., Kufe D. W., Spiegelman S., Gallo R. C., Gallagher R. E. 1976; Characterization of a virus (HL-23V) isolated from cultured acute myelogenous leukemia cells. Nature, London 260:266–268
    [Google Scholar]
  7. Deinhardt F., Bergholz C., Hunsmann G., Schneider J., Thiel H.J., Beug H., Schaffer W. 1978; Studies of simian sarcoma and simian sarcoma-associated virus. I. Analysis of viral structural proteins and preparation and characterization of antiserum specific for viral envelope components. Zeitschrift für Naturforschung 33c:969–980
    [Google Scholar]
  8. Gallagher R. E., Gallo R. C. 1975; Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells. Science 187:350–351
    [Google Scholar]
  9. Gallagher R., Salahuddin S. Z., Hall W. T., McCredie K. B., Gallo R. C. 1975; Growth and differentiation in culture of leukemic leukocytes from a patient with acute myelogenous leukemia and reidentification of type C virus. Proceedings of the National Academy of Sciences of the United States of America 72:4137–4141
    [Google Scholar]
  10. Hino S., Stephenson J. R., Aaronson S. A. 1975; Antigenic determinants of the 70000 molecular weight glycoprotein of woolly monkey type C RNA virus. Journal of Immunology 115:922–927
    [Google Scholar]
  11. Kawakami T. G., Huff S. D., Buckley P. M., Dungworth D. L., Snyder S. P., Gilden R. V. 1972; C-type virus associated with gibbon lymphosarcoma. Nature New Biology 235:170–171
    [Google Scholar]
  12. Okabe H., Gilden R. V., Hatanaka M., Stephenson J. R., Gallagher R. E., Gallo R. C., Tronick S. R., Aaronson S. A. 1976; Immunological and biochemical characterization of type C viruses isolated from cultured human AML cells. Nature, London 260:264–266
    [Google Scholar]
  13. Parks W. P., Scolnick E. M., Noon M. C., Watson C. J., Kawakami T. G. 1973; Radioimminoassay of mammalian type-C polypeptides. IV. Characterization of woolly monkey and gibbon viral antigens. International Journal of Cancer 12:129–137
    [Google Scholar]
  14. Reitz M. S., Miller N. R., Wong-Staal F., Gallagher R. E., Gallo R. C., Gillespie D. H. 1976; Primate type-C virus nucleic acid sequences (woolly monkey and baboon types) in tissues from a patient with acute myelogenous leukemia and in viruses isolated from cultured cells of the same patient. Proceedings of the National Academy of Sciences of the United States of America 73:2113–2117
    [Google Scholar]
  15. Smith R. G., Donehower L., Gallo R. C., Gillespie D. 1976; Rapid purification of 70S RNA from media of cells producing RNA tumor viruses. Journal of Virology 17:287–290
    [Google Scholar]
  16. Sun L., Kawakami T. G., Matoba S. I. 1978; Genomic stability of gibbon oncornavirus. Journal of Virology 28:767–771
    [Google Scholar]
  17. Teich N., Weiss R., Salahuddin Z., Gallagher R., Gillespie D., Gallo R. C. 1975; Infective transmission and characterization of a C-type virus released by cultured human myeloid leukemia cells. Nature, London 256:551–555
    [Google Scholar]
  18. Todaro G. J., Sherr C. J., Sen A., King N., Daniel M. D., Fleckenstein B. 1978; Endogenous New World primate type C viruses isolated from owl monkey (Aotus trivirgatus) kidney cell line. Proceedings of the National Academy of Sciences of the United States of America 75:1004–1008
    [Google Scholar]
  19. Tronick S. R., Stephenson J. R., Aaronson S. A., Kawakami T. G. 1975; Antigenic characterization of type C RNA virus isolates of gibbon apes. Journal of Virology 15:115–120
    [Google Scholar]
  20. Wolfe L. G., Smith R., Deinhardt F. 1972; Simian sarcoma virus type i (Lagothrix): focus assay and demonstration of nontransforming associated virus. Journal of the National Cancer Institute 48:1905–1908
    [Google Scholar]
  21. Wu A. M., Reitz M. S., Paran M., Gallo R. C. 1974; Mechanism of stimulation of murine type-C RNA tumor virus production by glucocorticoids: post-transcriptional effects. Journal of Virology 14:802–812
    [Google Scholar]
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