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Labelled oligomannosyl type glycans of E2 protein of Semliki Forest virus were liberated by treatment with endo-β-N-acetylglucosaminidase H, as well as by hydrazinolysis and analysed by paper chromatography. The protein-bound virus oligosaccharides were a mixture of Man7GlcNAc2, Man6GlcNAc2 and Man5GlcNAc2, and Man8GlcNAc2 also appeared to be present. P62 protein, the direct precursor of E2, was labelled by supplying infected BHK cells with 2-3H-mannose for 5 min. The oligomannosyl glycans of the P62 proteins formed were about two monosaccharide units larger than those of the mature virus E2 proteins.
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