1887

Abstract

SUMMARY

Labelled oligomannosyl type glycans of E2 protein of Semliki Forest virus were liberated by treatment with endo-β--acetylglucosaminidase H, as well as by hydrazinolysis and analysed by paper chromatography. The protein-bound virus oligosaccharides were a mixture of ManGlcNAc, ManGlcNAc and ManGlcNAc, and ManGlcNAc also appeared to be present. P62 protein, the direct precursor of E2, was labelled by supplying infected BHK cells with 2-H-mannose for 5 min. The oligomannosyl glycans of the P62 proteins formed were about two monosaccharide units larger than those of the mature virus E2 proteins.

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/content/journal/jgv/10.1099/0022-1317-47-2-525
1980-04-01
2023-02-06
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