RT Journal Article SR Electronic(1) A1 Bar-Joseph, M. A1 Salomon, R.YR 1980 T1 Heterologous Reactivity of Tobacco Mosaic Virus Strains in Enzyme-linked Immunosorbent Assays JF Journal of General Virology, VO 47 IS 2 SP 509 OP 512 DO https://doi.org/10.1099/0022-1317-47-2-509 PB Microbiology Society, SN 1465-2099, AB SUMMARY Enzyme-linked immunosorbent assay (ELISA) differentiated between the antigens of the type strains of tobacco mosaic virus (TMV) and those of the avocado isolate (TMV-A). The ELISA specificity in the heterologous antibody systems was affected mainly by the behaviour of the free phase (conjugate antibodies) whereas antibodies used for coating cross-reacted as in double-diffusion tests. Binding of bovine serum albumin to the TMV and TMV-A antibodies used for coating impaired their activity when tested in homologous reactions but did not make them completely specific in the heterologous system. The complexes of both TMV and TMV-A antigens bound to their homologous and heterologous coated antibodies were stable to acid dissociation. Some possible explanations of ELISA specificity are discussed., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-47-2-509