Monoclonal Antibodies to Influenza Matrix Protein: Detection of Low Levels of Matrix Protein on Abortively Infected Cells Free

Abstract

SUMMARY

We have quantified the appearance of matrix (M-) protein on P815 mastocytoma cells infected with type A influenza virus using a monoclonal antibody specific for -protein. In contrast to previously reported values, only low amounts (about 10 molecules/cell) of -protein appear on the cell surface up to 16 h p.i. Since P815 cells are excellent targets for cross-reactive T-cell lysis 3 to 5 h after virus infection, when only about 10 -protein sites are found on each cell surface, it appears less likely that the recognition of -protein can account for the cross-reactivity of cytotoxic T-lymphocytes for different type A influenza viruses.

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1980-04-01
2024-03-29
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