1887

Abstract

SUMMARY

An indirect sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect the baculovirus of in purified virus preparations and in extracts of larvae and adults collected in the field in Tonga. A 20 ng amount of purified virus was detected unequivocally but precise quantitative determinations of virus in insect extracts proved difficult because virus in solutions containing host protein gave lower absorbance values than a comparable purified virus standard. The implications for use of ELISA in ecological studies are discussed together with the choice of appropriate standards.

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/content/journal/jgv/10.1099/0022-1317-47-2-431
1980-04-01
2022-01-18
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