1887

Abstract

SUMMARY

An indirect sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect the baculovirus of in purified virus preparations and in extracts of larvae and adults collected in the field in Tonga. A 20 ng amount of purified virus was detected unequivocally but precise quantitative determinations of virus in insect extracts proved difficult because virus in solutions containing host protein gave lower absorbance values than a comparable purified virus standard. The implications for use of ELISA in ecological studies are discussed together with the choice of appropriate standards.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-47-2-431
1980-04-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/47/2/JV0470020431.html?itemId=/content/journal/jgv/10.1099/0022-1317-47-2-431&mimeType=html&fmt=ahah

References

  1. Catley A. 1969; The coconut rhinoceros beetle, Oryctes rhinoceros (L). PANS 15:18–30
    [Google Scholar]
  2. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  3. Crawford A. M., Faulkner P., Kalmakoff J. 1978; Comparison of solid-phase radio immuno assays for baculoviruses. Applied and Environmental Microbiology 36:18–24
    [Google Scholar]
  4. Flegg C. L., Clark M. F. 1979; The detection of apple chlorotic leafspot virus by a modified procedure of enzyme-linked immunosorbent assay (ELISA). Annals of Applied Biology 91:61–65
    [Google Scholar]
  5. Kelly D. C., Edwards M-L., Evans H. F., Robertson J. S. 1978a; The use of the enzyme-linked immunosorbent assay to detect a nuclear polyhedrosis virus in Heliothis armigera larvae. Journal of General Virology 40:465–469
    [Google Scholar]
  6. Kelly D. C., Edwards M.-L., Robertson J. S. 1978b; The use of enzyme-linked immunosorbent assay to detect, and discriminate between small iridescent viruses. Annals of Applied Biology 90:369–374
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  8. Young E. C. 1974; The epizootiology of two pathogens of the coconut palm rhinoceros beetle. Journal of Invertebrate Pathology 24:82–92
    [Google Scholar]
  9. Zelazny B. 1971; Experiments with Rhabdionvirus oryctes. In UN/SPC Rhinoceros Beetle Project Report June 1970-May 1971, pp 42–125
    [Google Scholar]
  10. Zelazny B. 1976; Transmission of baculovirus in populations of Oryctes rhinoceros. Journal of Invertebrate Pathology 27:221–227
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-47-2-431
Loading
/content/journal/jgv/10.1099/0022-1317-47-2-431
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error