%0 Journal Article %A Wiegers, K. J. %A Drzeniek, R. %T Preparative Isoelectric Focusing of Poliovirus Polypeptides in Urea-Sucrose Gradients %D 1980 %J Journal of General Virology, %V 47 %N 2 %P 423-430 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-47-2-423 %I Microbiology Society, %X SUMMARY Isoelectric focusing in urea using small density gradient columns for dissociated poliovirus resulted in a complete separation of the four virus polypeptides. Identification and purity of VP1, 2, 3 and 4 was shown by SDS-polyacrylamide gel electrophoresis. The isoelectric points were determined and compared with the values obtained in gels. The recovery of the individual polypeptides was about 80%. Up to 500 µg of poliovirus per tube could thus be separated in a one-step procedure giving pure and SDS-free poliovirus polypeptides. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-47-2-423