1887

Abstract

SUMMARY

Synthesis of vesicular stomatitis virus RNA and protein is almost completely inhibited in HeLa cells treated with relatively high doses of human fibroblast interferon. With lower concentrations of interferon virus replication is inhibited, but near normal amounts of virus RNA are found in cells infected at a m.o.i. of 10. All the virus RNA species are found in these cells with the exception of genomic size RNA. In contrast, synthesis of all the virus proteins is equally inhibited in proportion to the interferon concentration used to treat the cells. This inhibition is due to a decline in the rate of protein synthesis, which occurs in interferon-treated cells sooner after infection than in untreated cells. The decreased rate of protein synthesis is accompanied by a change in the polysome pattern of infected cells, characterized by polysome run-off and increase in 80S ribosomes. At the same time, a larger proportion of the virus poly(A)-containing RNA is not associated with polysomes in interferon-treated cells than in control cells. The non-polysomal virus RNA has a sedimentation rate identical with that of polysomal virus RNA. Possible causes for the decline in the rate of protein synthesis observed in interferon-treated cells are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-47-2-373
1980-04-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/47/2/JV0470020373.html?itemId=/content/journal/jgv/10.1099/0022-1317-47-2-373&mimeType=html&fmt=ahah

References

  1. Baglioni C. 1979; Interferon induced enzymatic activities and their role in the antiviral state. Cell 17:255–264
    [Google Scholar]
  2. Banerjee A. K., Rhodes D. P. 1973; In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerases of vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 70:3566–3570
    [Google Scholar]
  3. Baxt B., Sonnabend J. A., Bablanian R. 1977; Effects of interferon on vesicular stomatitis virus transcription and translation. Journal of General Virology 35:325–334
    [Google Scholar]
  4. Clinton G. M., Little S. P., Hagan R. S., Huang A. S. 1978; The matrix (M) protein of vesicular stomatitis virus regulates transcription. Cell 15:1455–1462
    [Google Scholar]
  5. Colby C., Duesberg P. H. 1969; Double-stranded RNA in vaccinia-virus infected cells. Nature, London 222:940–944
    [Google Scholar]
  6. Desrosiers R. C., Lengyel P. 1977; Impairment of reovirus mRNA cap méthylation in interferon-treated L cells. Federation Proceedings 36:812
    [Google Scholar]
  7. Duesberg P. H., Colby C. 1969; On the biosynthesis and structure of double-stranded RNA in vaccinia-virus infected cells. Proceedings of the National Academy of Sciences of the United States of America 64:396–403
    [Google Scholar]
  8. Gupta S. L. 1979; Specific protein phosphorylation in interferon-treated uninfected and virus-infected mouse L929 cells: enhancement by double-stranded RNA. Journal of Virology 29:301–311
    [Google Scholar]
  9. Kerr I. M., Brown R. E. 1978; pppA2′ p5′ A2′ p5′ A: An inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells. Proceedings of the National Academy of Sciences of America 75:256–260
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Laskey R. A., Mills D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  12. Lodish H. F. 1976; Translational control of protein synthesis. Annual Review of Biochemistry 45:39–72
    [Google Scholar]
  13. Manders E. K., Tilles J. G., Huang A. S. 1972; Interferon-mediated inhibition of virion-directed transcription. Virology 49:573–581
    [Google Scholar]
  14. Marcus P. L., Engelhardt D. L., Hung J. M., Sekellick M. J. 1971; Interferon action: inhibition of VSV RNA synthesis induced by virion-bound polymerase. Science 174:593–598
    [Google Scholar]
  15. Marcus P. L., Sekellick M. J. 1978; Interferon action. III. The rate of primary transcription of vesicular stomatitis virus is inhibited by interferon action. Journal of General Virology 38:391–408
    [Google Scholar]
  16. Metz D. H., Esteban M. 1972; Interferon inhibits viral protein synthesis in L cells infected with vaccinia virus. Nature, London 238:385–388
    [Google Scholar]
  17. Metz D. H., Esteban M., Danielescu G. 1975; The effect of interferon on the formation of virus polyribosomes in L-cells infected with vaccinia virus. Journal of General Virology 27:197–209
    [Google Scholar]
  18. Muthukrishnan S., Moss B., Cooper J. A., Maxell E. S. 1978; Influence of 5′-terminal structure on the initiation of translation of vaccinia virus mRNA. Journal of Biological Chemistry 253:1710–1715
    [Google Scholar]
  19. Repik P., Flamand A., Bishop D. H. L. 1974; Effect of interferon upon the primary and secondary transcription of VSV and influenza viruses. Journal of Virology 14:1169–1178
    [Google Scholar]
  20. Rose J. K., Lodish H. F. 1976; Translation in vitro of vesicular stomatitis virus mRNA lacking 5′-terminal 7-methylguanosine. Nature, London 262:32–37
    [Google Scholar]
  21. Thacore H. R. 1978; Effect of interferon on transcription and translation of vesicular stomatitis virus in human and simian cell cultures. Journal of General Virology 41:421–426
    [Google Scholar]
  22. Weber L. A., Feman E., Baglioni C. 1975; A cell-free system from HeLa cells active in initiation of protein synthesis. Biochemistry 14:5315–5321
    [Google Scholar]
  23. Weber L. A., Simili I., Baglioni C. 1979; Binding of viral and cellular messenger RNA’s to ribosomes in eukaryotic cell extracts. Methods in Enzymology 60:351–360
    [Google Scholar]
  24. Wertz G. W. 1978; Isolation of possible replicative intermediate structures from vesicular stomatitis virus-infected cells. Virology 85:271–285
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-47-2-373
Loading
/content/journal/jgv/10.1099/0022-1317-47-2-373
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error