In Vero cells incubated at 40°C or treated with azetidine at 37°C, synthesis of a polypeptide (‘C’) of apparent mol. wt. 66000 was stimulated. It was not phosphorylated and was found in the cytoplasmic fraction of cell lysates. In cells infected with herpes simplex virus type 1 (HSV-1) in the presence of azetidine, synthesis of cellular proteins, including polypeptide C, was suppressed and infected cell polypeptides ICP 4, 0, 22 and 27 (apparent mol. wt. 170000, 120000, 75000 and 60000, respectively) were made. All were phosphorylated and accumulated in the nucleus. Messenger RNA for the same four polypeptides was made in cells infected in the presence of cycloheximide. Thus, ICP 22 is distinct from cellular polypeptide C and is probably a virus-specific α polypeptide, although it differs from α ICP 4, 0 and 27 in that its rate of synthesis does not decline rapidly when later polypeptides are produced. It is modified after synthesis in at least two steps, the second of which may require a later virus-specific polypeptide. In cells infected with HSV-2 the synthesis of a polypeptide analogous to ICP 22 could not be detected.
CourtneyR. J.,
PowellK. L.1975; Immunological and biochemical characterization of polypeptides induced by herpes simplex virus types 1 and 2. In Proceedings of the Symposium on Herpes Viruses and Oncogenesis vol 2 pp 63–73 Edited by
de ThèG.,
EpsteinM. A.,
Zur HausenH.
Lyon: International Agency for Research on Cancer;
FenwickM. L.,
WalkerM. J.,
PetkevichJ. M.1978; On the association of virus proteins with nuclei of cells infected with herpes simplex virus. Journal of General Virology 39:519–529
HonessR. W.,
RoizmanB.1974; Regulation of herpes virus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. Journal of Virology 14:8–19
HonessR. W.,
RoizmanB.1975; Regulation of herpesvirus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral polypeptides. Proceedings of the National Academy of Sciences of the United States of America 72:1276–1280
KozakM.,
RoizmanB.1974; Regulation of herpes virus macromolecular synthesis: nuclear retention of non -translated viral RNA sequences. Proceedings of the National Academy of Sciences of the United States of America 71:4322–4326
MorseL. S.,
PereiraL.,
RoizmanB.,
SchafferP. A.1978; Anatomy of herpes simplex virus DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 × HSV-2 recombinants. Journal of Virology 26:389–410
PereiraL.,
WolffM. H.,
FenwickM. L.,
RoizmanB.1977; Regulation of herpesvirus macromolecular synthesis. V. Properties of cl polypeptides made in HSV-1 and HSV-2 infected cells. Virology 77:733–749
PrestonC. M.1979; Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wildtype virus or the temperature-sensitive mutant tsK. Journal of Virology 29:275–284
PrestonV. G.,
DavisonA. J.,
MarsdenH. S.,
TimburyM. C.,
Subak-SharpeJ. H.,
WilkieN. M.1978; Recombinants between herpes simplex virus types 1 and 2: analyses of genome structures and expression of immediate early polypeptides. Journal of Virology 28:499–517