The rates of virus RNA synthesis and virion accumulation were investigated in brome mosaic virus-infected barley protoplasts. Single-stranded virus RNAs could be detected as early as 6 h after inoculation. Only RNA components 1 and 2 were detected at this time, suggesting that their synthesis is initiated relatively early in infection. The RNAs were synthesized at similar rates from 16 to 35 h post inoculation with maximal synthesis until approx. 25 h after inoculation. Double-stranded replicative forms of the four virus RNAs were observed. Their synthesis was first detectable at 6 h post inoculation and followed a time course similar to that of the single-stranded RNA species. Analysis of RNA encapsidation and infectivity assays of protoplast homogenates revealed that virion formation was greatest between 10 and 25 h after inoculation. All four RNAs were present in virions at 10 h post inoculation. Particles containing RNA 3 and RNA 4 accumulated at a faster rate than particles containing RNA 1 or RNA 2.
BancroftJ. B.,
MotoyoshiF.,
WattsJ. W.,
DawsonJ. R. O.1975; Cowpea chlorotic mottle and brome mosaic viruses in tobacco protoplasts. In Modification of the Information Content of Plant Cells pp 133–160 Edited by
MarkhamR.,
DaviesD. R.,
HopwoodD. A.,
HorneR. W.
Amsterdam: North-Hoiland;
DaviesJ. W.1976; The multipartite genome of brome mosaic virus: aspects of in vitro translation and RNA structure. Annales de Microbiologie (Institut Pasteur) 127A:131–142
HardyS. F.,
GermanT. L.,
Loesch-FriesL. S.,
HallT. C.1979; A highly active, template-specific RNA-dependent RNA polymerase from brome mosaic virus-infected barley leaves. Proceedings of the National Academy of Sciences of the United States of America 76:4956–4960
KohlR. J.,
HallT. C.1977; Loss of infectivity of brome mosaic virus RNA after chemical modification of the 3′ or 5′ terminus. Proceedings of the National Academy of Sciences of the United States of America 74:2682–2686
KummertJ.,
SemalJ.1977; Polyacrylamide gel electrophoresis of the RNA products labeled in vitro by extracts of leaves infected with brome mosaic virus. Virology 60:390–397
MotoyoshiF.,
BancroftJ. B.,
WattsJ. W.1974; The infection of tobacco protoplasts with a variant of brome mosaic virus. Journal of General Virology 25:31–36
OkunoT.,
FurusawaL.1978; Factors influencing the infection of barley mesophyll protoplasts with brome mosaic virus RNA. Journal of General Virology 41:63–75
PeacockA. C.,
DingmanC. W.1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
PyneJ. W.,
HallT. C.1979; Efficient ribosome binding of brome mosaic virus (BMV) RNA 4 contributes to its ability to outcompete the other BMV RNAs for translation. Intervirology 11:23–29
ShepardJ. F.1972; Gel-diffusion Methods for the Serological Detection of Potato Viruses X, S, and M. Montana Agricultural Experiment Station Bulletin662 Bozeman, Montana: Montana State University;
ShihD. S.,
KaesbergP.1973; Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo. Proceedings of the National Academy of Sciences of the United States of Americano 70:1799–1803
ShihD. S.,
KaesbergP.1976; Translation of the RNAs of brome mosaic virus: the monocistronic nature of RNA 1 and RNA 2. Journal of Molecular Biology 103:77–88