1887

Abstract

SUMMARY

A new protein has been detected in the nuclei of vaccinia virus-infected cells. This protein has an apparent mol. wt. of 28000 (VP28) on SDS-polyacrylamide gels and has been detected in Triton X-100-treated nuclei of infected BSC-40, L-929 and CVC cells. Within the infected cells, VP28 was synthesized maximally at 1 to 2 h p.i. in the cytoplasm and accumulated in the nuclei at 4 to 5 h p.i. The appearance of VP28 was not affected by cytosine arabinoside (25 g/ml), an inhibitor of virus DNA synthesis, or rifampicin (100 g/ml), an inhibitor of vaccinia assembly, but was inhibited by irradiation of the infecting virions; thus classifying it as an early vaccinia virus gene product. Nuclear-cytoplasmic mixing experiments suggested that the nuclear location of VP28 was not an artefact of the cell fractionation techniques employed. VP28 did not appear to be phosphorylated.

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1980-04-01
2024-12-07
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References

  1. Birnie G. D. 1978; Isolation of nuclei from animal cells in culture. In Methods in Cell Biology pp 13–26 Edited by Prescott D. M. New York: Academic Press;
    [Google Scholar]
  2. Bolden A., Pendrali-Noy G., Weissbach A. 1979; Vaccinia virus infection of HeLa cells. II. Disparity between cytoplasmic and nuclear viral-specific RNA. Virology 94:138–145
    [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  4. Gafford L. G., Randall C. C. 1976; Virus-specific RNA and DNA in nuclei of cells infected with fowl-poxvirus. Virology 69:1–14
    [Google Scholar]
  5. Goodpasture E. W., Anderson K. 1962; Isolation of a wild avian poxvirus inducing both cytoplasmic and nuclear inclusions. American Journal of Pathology 40:437–454
    [Google Scholar]
  6. Hardy C. L., Randall C. C., Gafford L. G. 1978; A viral-induced protein in the nuclei of cells infected with fowlpox virus. Virology 84:236–241
    [Google Scholar]
  7. Hightower L. E., Smith M. D. 1978; Effects of canavanine on protein metabolism in Newcastle disease virus-infected and uninfected chicken embryo cells. In Negative Strand Viruses and the Host Cell PP 395–405 Edited by Mahy B. W. J., Barry R. D. New York: Academic Press;
    [Google Scholar]
  8. Hruby D. E., Guarino L. A., Rates J. R. 1979a; Vaccinia virus replication. E Requirement for the host cell nucleus. Journal of Virology 29:705–715
    [Google Scholar]
  9. Hruby D. E., Lynn D. L., Kates J. R. 1979b; Vaccinia virus replication requires active participation of the host cell transcriptional apparatus. Proceedings of the National Academy of Sciences of the United States of America 76:1887–1890
    [Google Scholar]
  10. Kelley P. M., Schlesinger M. J. 1978; The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts. Cell 15:1277–1286
    [Google Scholar]
  11. Lacolla P., Weissbach A. 1975; Vaccinia virus infection of HeLa cells. I. Synthesis of vaccinia DNA in host cell nuclei. Journal of Virology 15:305–315
    [Google Scholar]
  12. Moss B. 1974; Reproduction of poxviruses. In Comprehensive Virology vol 3 pp 405–474 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  13. Nowakowski M., Bauer W., Kates J. 1978a; Characterization of a DNA-binding phosphoprotein from vaccinia virus replication complex. Virology 86:217–225
    [Google Scholar]
  14. Nowakowski M., Kates J., Bauer W. 1978b; Isolation of two DNA-binding proteins from the intracellular replication complex of vaccinia virus. Virology 84:260–267
    [Google Scholar]
  15. Opperman H., Koch G. 1976; On the regulation of protein synthesis in vaccinia virus infected cells. Journal of General Virology 32:261–273
    [Google Scholar]
  16. Pennington T. H. 1974; Vaccinia virus polypeptide synthesis: sequential appearance and stability of pre- and post-replicative polypeptides. Journal of General Virology 25:433–444
    [Google Scholar]
  17. Pennington T. H., Follett E. A. C. 1974; Vaccinia virus replication in enucleate BSC-1 cells: particle production and synthesis of viral DNA and proteins. Journal of Virology 13:488–493
    [Google Scholar]
  18. Portner A., Pridgen C. 1975; Viral replication. In Viral Immunology and Immimopathy pp 27–42 Edited by Notkins A. New York: Academic Press;
    [Google Scholar]
  19. Rosemond H., Moss B. 1973; Phosphoprotein component of vaccinia virions. Journal of Virology 11:961–970
    [Google Scholar]
  20. Soloski M. J., Esteban M., Holowczak J. A. 1978; DNA-binding proteins in the cytoplasm of vaccinia virus infected mouse L-cells. Journal of Virology 25:263–273
    [Google Scholar]
  21. Studier F. W. 1973; Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
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