%0 Journal Article %A Clark, Donald P. %A Dougherty, Robert M. %T Detection of Avian Oncovirus Group-specific Antigens by the Enzyme-linked Immunosorbent Assay %D 1980 %J Journal of General Virology, %V 47 %N 2 %P 283-291 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-47-2-283 %I Microbiology Society, %X SUMMARY A three-step sandwich enzyme-linked immunosorbent assay was developed for the detection of avian oncovirus group-specific (gs) antigens. The assay procedure was to coat the wells of microtitre plates with hamster anti-gs IgG, react with crude or purified antigen and finally with hamster anti-gs IgG linked to horseradish peroxidase. The sensitivity was 8 picograms (pg) of input avian myeloblastosis virus (AMV) protein, with negligible background. As the ELISA takes less than 2 h to perform, large-scale screening for infected birds is feasible. A blocking assay was also developed for detecting anti-gs antibodies by adding unlabelled antiserum after the antigen step. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-47-2-283