@article{mbs:/content/journal/jgv/10.1099/0022-1317-47-1-89, author = "Marcus, Philip I. and Sekellick, Margaret J.", title = "Interferon Induction by Viruses. III. Vesicular Stomatitis Virus: Interferon-inducing Particle Activity Requires Partial Transcription of Gene N", journal= "Journal of General Virology", year = "1980", volume = "47", number = "1", pages = "89-96", doi = "https://doi.org/10.1099/0022-1317-47-1-89", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-47-1-89", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY We have measured the interferon-inducing particle (i.f.p.) activity of a ts mutant, G11(I), of vesicular stomatitis virus (VSV) and a non-ts revertant, R1 (T1026) in ‘aged’ chick embryo cells and mouse L(Y) cells at 40.5 and 37.5°C, respectively. Our results suggest that a single i.f.p. suffices to induce a quantum yield of interferon and that there are several times more i.f.p. than plaque-forming particles (p.f.p.) in stock preparations of VSV. Furthermore, while virus replication or amplified RNA synthesis is not required for a particle of VSV to induce interferon, there is a requirement for primary transcription. About one-tenth of the genome must remain intact and be transcribed to synthesize an interferon-inducer moiety. (This represents transcription of about two-thirds of the N protein gene.) We conclude that VSV does not contain a pre-formed inducer of interferon and propose a model for its formation. We suggest that there is a cumulative loss of N (and/or NS and L) protein from the ribonucleoprotein complex during primary transcription, leading ultimately to extensive base-pairing between the genome RNA and its complementary transcript. We suggest that the dsRNA thus formed constitutes the interferon inducer moiety of VSV.", }