Proteins associated with 36S virus RNA from Vero cells infected with San Miguel sea lion virus, type 2 (SMSV-2), were labelled with 125I. One protein, VPg, remained linked to RNA when subjected to deproteinization techniques. VPg labelled with 32P was observed on 36S RNA from purified virions; the quantity of label was compatible with two phosphates per genome. The estimated mol. wt. of SMSV-2 VPg was 15000.
AmbrosV.,
BaltimoreD.1978; Protein is linked to the 5′ end of poliovirus RNA by a phosphodiester linkage to tyrosine. Journal of Biological Chemistry 253:5263–5266
BakerE. E.,
SommerH.,
FosterL. E.,
MeyerE.,
MeyerK. F.1952; Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis
. Journal of Immunology 68:131–145
BurkeR. L.,
BauerW. R.1977; The properties of native and denatured DNA in buoyant rubidium trichloroacetate at neutral pH. Nucleic Acids Research 4:1891–1909
DenoyaC. D.,
ScodellerE. A.,
VasquezC.,
La TorreJ. L.1978; Foot and mouth disease virus. II Endoribonuclease activity within purified virions. Virology 89:67–74
EhresmannD. W.1978; Studies on calicivirus genomic replication and transcription: characterization of RNA from virions and infected cells. Ph.D. thesis University of California;
EhresmannD. W.,
SchafferF. L.1979; Calicivirus intracellular RNA: fractionation of 18 to 22 S RNA, and lack of typical 5′-methylated caps on 36 S and 22 S San Miguel sea lion virus RNAs. Virology 95:251–255
FlaneganJ. B.,
PetterssonR. F.,
AmbrosV.,
HewlettM. J.,
BaltimoreD.1977; Covalent linkage of a protein to a defined nucleotide sequence at the 5′-terminus of virion and replicative intermediate RNAs of poliovirus. Proceedings of the National Academy of Sciences of the United States of America 74:691–695
GoliniF.,
NomotoA.,
WimmerE.1978; The genome-linked protein of picornaviruses. IV. Differences in the VPg’s of encephalomyocarditis virus and poliovirus as evidence that the genome-linked proteins are virus-coded. Virology 89:112–118
LeeY. F.,
NomotoA.,
DetjenB. M.,
WimmerE.1977; A protein covalently linked to poliovirus genome RNA. Proceedings of the National Academy of Sciences of the United States of America 74:59–63
NomotoA.,
DetjenB.,
PozzattiR.,
WimmerE.1977a; The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature, London 268:208–213
NomotoA.,
KitamuraN.,
GoliniF.,
WimmerE.1977b; The 5′-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. Proceedings of the National Academy of Sciences of the United States of America 74:5345–5349
SchafferF. L.,
SoergelM. E.1976; Single major polypeptide of a calicivirus: characterization by polyacrylamide gel electrophoresis and stabilization of virions by cross-linking with dimethyl suberimidate. Journal of Virology 19:925–931