1887

Abstract

SUMMARY

Proteins associated with 36S virus RNA from Vero cells infected with San Miguel sea lion virus, type 2 (SMSV-2), were labelled with I. One protein, VPg, remained linked to RNA when subjected to deproteinization techniques. VPg labelled with P was observed on 36S RNA from purified virions; the quantity of label was compatible with two phosphates per genome. The estimated mol. wt. of SMSV-2 VPg was 15000.

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1980-03-01
2022-01-20
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References

  1. Ambros V., Baltimore D. 1978; Protein is linked to the 5′ end of poliovirus RNA by a phosphodiester linkage to tyrosine. Journal of Biological Chemistry 253:5263–5266
    [Google Scholar]
  2. Baker E. E., Sommer H., Foster L. E., Meyer E., Meyer K. F. 1952; Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis . Journal of Immunology 68:131–145
    [Google Scholar]
  3. Black D. N., Burroughs J. N., Harris T. J. R., Brown F. 1978; The structure and replication of calicivirus RNA. Nature, London 274:614–615
    [Google Scholar]
  4. Burke R. L., Bauer W. R. 1977; The properties of native and denatured DNA in buoyant rubidium trichloroacetate at neutral pH. Nucleic Acids Research 4:1891–1909
    [Google Scholar]
  5. Burke R. L., Anderson P. J., Bauer W. R. 1978; Resolution of single- and double-stranded RNAs in buoyant cesium trichloroacetate. Analytical Biochemistry 36:264–270
    [Google Scholar]
  6. Burroughs J. N., Brown F. 1974; Physico-chemical evidence for the re-classification of the caliciviruses. Journal of General Virology 22:281–286
    [Google Scholar]
  7. Burroughs J. N., Brown F. 1978; Presence of a covalently linked protein on calicivirus RNA. Journal of General Virology 41:443–446
    [Google Scholar]
  8. Denoya C. D., Scodeller E. A., Vasquez C., La Torre J. L. 1978; Foot and mouth disease virus. II Endoribonuclease activity within purified virions. Virology 89:67–74
    [Google Scholar]
  9. Ehresmann D. W. 1978; Studies on calicivirus genomic replication and transcription: characterization of RNA from virions and infected cells. Ph.D. thesis University of California;
    [Google Scholar]
  10. Ehresmann D. W., Schaffer F. L. 1977; RNA synthesized in calicivirus-infected cells is atypical of picornaviruses. Journal of Virology 22:572–576
    [Google Scholar]
  11. Ehresmann D. W., Schaffer F. L. 1979; Calicivirus intracellular RNA: fractionation of 18 to 22 S RNA, and lack of typical 5′-methylated caps on 36 S and 22 S San Miguel sea lion virus RNAs. Virology 95:251–255
    [Google Scholar]
  12. Flanegan J. B., Pettersson R. F., Ambros V., Hewlett M. J., Baltimore D. 1977; Covalent linkage of a protein to a defined nucleotide sequence at the 5′-terminus of virion and replicative intermediate RNAs of poliovirus. Proceedings of the National Academy of Sciences of the United States of America 74:691–695
    [Google Scholar]
  13. Fretz M., Schaffer F. L. 1978; Calicivirus proteins in infected cells: evidence for a capsid polypeptide precursor. Virology 89:318–321
    [Google Scholar]
  14. Golini F., Nomoto A., Wimmer E. 1978; The genome-linked protein of picornaviruses. IV. Differences in the VPg’s of encephalomyocarditis virus and poliovirus as evidence that the genome-linked proteins are virus-coded. Virology 89:112–118
    [Google Scholar]
  15. Harrison B. D., Barker H. 1978; Protease-sensitive structure needed for infectivity of nepovirus RNA. Journal of General Virology 40:711–715
    [Google Scholar]
  16. Hruby D. E., Roberts W. K. 1978; Encephalomyocarditis virus RNA. III. Presence of a genome-associated protein. Journal of Virology 25:413–415
    [Google Scholar]
  17. Lee Y. F., Nomoto A., Detjen B. M., Wimmer E. 1977; A protein covalently linked to poliovirus genome RNA. Proceedings of the National Academy of Sciences of the United States of America 74:59–63
    [Google Scholar]
  18. Mayo M. A., Barker H., Harrison B. D. 1979; Evidence for a protein covalently linked to tobacco ringspot virus RNA. Journal of General Virology 43:735–740
    [Google Scholar]
  19. Nomoto A., Detjen B., Pozzatti R., Wimmer E. 1977a; The location of the polio genome protein in viral RNAs and its implication for RNA synthesis. Nature, London 268:208–213
    [Google Scholar]
  20. Nomoto A., Kitamura N., Golini F., Wimmer E. 1977b; The 5′-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. Proceedings of the National Academy of Sciences of the United States of America 74:5345–5349
    [Google Scholar]
  21. Sangar D. V., Rowlands D. J., Harris T. J. R., Brown F. 1977; Protein covalently linked to foot-and-mouth disease virus RNA. Nature, London 268:648–650
    [Google Scholar]
  22. Schaffer F. L., Soergel M. E. 1973; Biochemical and biophysical characterization of calicivirus isolates from pinnipeds. Intervirology 1:210–219
    [Google Scholar]
  23. Schaffer F. L., Soergel M. E. 1976; Single major polypeptide of a calicivirus: characterization by polyacrylamide gel electrophoresis and stabilization of virions by cross-linking with dimethyl suberimidate. Journal of Virology 19:925–931
    [Google Scholar]
  24. Scott J. F., Kuhns V. L. 1972; Isolation of ribonucleic acid from animal tissue by use of chaotropic agents. Analytical Biochemistry 47:471–480
    [Google Scholar]
  25. Yogo Y., Wimmer E. 1973; Poly (A) and poly(U) in poliovirus double stranded RNA. Nature, London 242171–174
    [Google Scholar]
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