RT Journal Article SR Electronic(1) A1 Brown, Glenn Earl A1 Simon, Edward H. A1 Chung, CarolynYR 1980 T1 Interferon Production by Individual L Cells JF Journal of General Virology, VO 47 IS 1 SP 171 OP 182 DO https://doi.org/10.1099/0022-1317-47-1-171 PB Microbiology Society, SN 1465-2099, AB SUMMARY Using a protected centre technique in which agarose prevents the diffusion of interferon from individual producing cells, we have shown that essentially every cell in a monolayer of mouse L cells can be induced to produce interferon by infection with Newcastle disease virus (NDV). The amount of interferon produced by individual cells appeared to be highly variable, even when cloned cells and viruses were used. U.v.-irradiated virus lost its capacity to induce interferon in L cells and to infect chick embryo fibroblasts at the same rate. A small proportion of cells (1 × 10−6 to 10 × 10−6) appeared to produce interferon constitutively. This fraction was increased threefold by u.v. irradiation of the cells, and up to 10-fold by exposing cells to the mutagen ethyl methane sulphonate., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-47-1-171