Ultrastructural changes developing in chick embryo fibroblast cultures infected with a wild-type strain of fowl plague virus (FPV) or one of six FPV temperature-sensitive () mutants belonging to different complementation groups were studied. Cells infected with wild-type FPV and incubated at optimal (36 °C) or nonpermissive temperature (42 °C) displayed changes similar to those described for orthomyxoviruses. The same patterns of changes were observed at 36 °C in cells infected with mutants belonging to five of the complementation groups. Mutant 303, possessing mutation-altered haemagglutinin, induced at 36 °C the formation of virions carrying a considerably reduced number of spikes on their surfaces. At 42 °C, cells infected with mutant 131, with a defective primary transcription stage, showed no morphological changes and no formation of electron-dense inclusions. Cells infected with mutants with defective secondary transcription or replication displayed nuclear inclusions but no formation of filamentous cytoplasmic structures or virions. Mutant 5 with defective late morphogenesis induced formation of considerably enhanced numbers of nuclear inclusions.


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