Enzyme activities of particulate and solubilized RNA-dependent RNA polymerase preparations from tobacco plants infected with 10 ts mutants of alfalfa mosaic virus (AMV), having their mutations in each of the three parts of the virus genome, were found to behave similarly with respect to temperature as compared to preparations obtained from plants infected with wild type AMV or from uninfected plants. Thus it is very unlikely that a virus-specific protein is involved in these in vitro RNA polymerase activities.
BishopD. H. L.,
FlamandA.1975; Transcription processes of animal RNA viruses. In Control Processes in Virus Multiplication, Symposium 25 of the Society for General Microbiology pp 95–152 Edited by
BurkeD. C.,
RussellW. C.
Cambridge: Cambridge University Press;
BolJ. F.,
Clerx-Van HaasterC. M.,
WeeningC. J.1976; Host and virus specific RNA polymerases in alfalfa mosaic virus infected tobacco. Annales de Microbiologie 127A:183–192
ClerxC. M.,
BolJ. F.1978; Properties of solubilized RNA-dependent RNA polymerase from alfalfa mosaic virus-infected and healthy tobacco plants. Virology 91:453–463
EoyangL.,
AugustJ. T.1974; Reproduction of RNA bacteriophages. In Comprehensive Virology vol 2 pp 1–59 Edited by
FraenkelConratH.,
WagnerR. R.
New York and London: Plenum Press;
FlaneganJ. B.,
BaltimoreD.1979; Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide. Journal of Virology 29:352–360
IkegamiM.,
Fraenkel-ConratH.1978; RNA-dependent RNA polymerase of tobacco plants. Proceedings of the National Academy of Sciences of the United States of America 75:2122–2124
KumarasamyR.,
SymonsR. H.1978; Extensive purification of the RNA replicase induced by cucumber mosaic virus. In International Virology IV, Abstracts of the Fourth International Congress for Virology The Hague, The Netherlands: p 317
ScallaR.,
RomaineP.,
AsselinA.,
RigaudJ.,
ZaitlinM.1978; An in vivo study of a nonstructural polypeptide synthesized upon TMV infection and its identification with a polypeptide synthesized in vitro from TMV RNA. Virology 91:182–193
Van Vloten-DottngL.,
JasparsE. M. J.1977; Plant covirus systems, three component systems. In Comprehensive Virology vol II pp 1–53 Edited by
Fraenkel-ConratH.,
WagnerR. R.
New York and London: Plenum Press;
Van Vloten-DotingL.,
HasratJ. A.,
OosterwijkE.,
Van’TsantP.,
SchoenM. A.,
RoosienJ.1979; Description and complementation analysis of 13 temperature sensitive mutants of alfalfa mosaic virus. Journal of General Virology 46: (this part)
ZabelP.,
Jongen-NevenI.,
Van KammenA.1979; In vitro replication of cowpea mosaic virus RNA. III. Template recognition by cowpea mosaic virus RNA replicase. Journal of Virology 29:21–33