1887

Abstract

SUMMARY

Thirteen thermosensitive () mutants of alfalfa mosaic virus (AMV), a virus with a tripartite genome, are described. Eight of these mutants were spontaneous, one was induced with HNO and four were induced by u.v. irradiation of one purified component. Using supplementation tests six mutations were located on top component (Tb), three on middle component (M) and four on bottom component (B). Complementation tests with mutants with defects on the same component showed that none of the mutants on Tb could complement each other; the three mutants on M could be subdivided into two complementation groups, while only one pair of mutants showed complementation from the four mutants on B. It was demonstrated that the coat proteins from the six mutants on Tb were not able to activate the AMV genome at 30 °C in tobacco.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-46-2-415
1980-02-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/46/2/JV0460020415.html?itemId=/content/journal/jgv/10.1099/0022-1317-46-2-415&mimeType=html&fmt=ahah

References

  1. Bancroft J. B., Lane L. C. 1973; Genetic analysis of cowpea chlorotic mottle and brome mosaic viruses. Journal of General Virology 19:381–389
    [Google Scholar]
  2. Bancroft J. B., Rees M. W., Dawson J. R. O., McLean O. D., Short M. N. 1972; Some properties of a temperature sensitive mutant of cowpea chlorotic mottle virus. Journal of General Virology 16:69–81
    [Google Scholar]
  3. Bol J. F., Van Vloten-Doting L. 1973; Function of top component a RNA in the initiation of infection by alfalfa mosaic virus. Virology 51:102–108
    [Google Scholar]
  4. Bol J. F., Van Vloten-Doting L., Jaspars E. M. J. 1971; A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85
    [Google Scholar]
  5. Bol J. F., Brederode F. Th., Janze G. C., Rauh D. K. 1975; Studies on sequence homology between the RNA’s of alfalfa mosaic virus. Virology 65:1–15
    [Google Scholar]
  6. Bos L. 1969; Experiences with a collection of plant viruses in leaf material dried and stored over calcium chloride, and a discussion of literature on virus preservation. Mededelingen Rijksfaculteit Landbouw-wetenschappen Gent 34:875–887
    [Google Scholar]
  7. Cooper P. D. 1977; Genetics of picornaviruses. In Comprehensive Virology vol 9 pp 133–207 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  8. Crick F. H. C., Orgel L. E. 1964; The theory of interallelic complementation. Journal of Molecular Biology 8:161–165
    [Google Scholar]
  9. Dawson W. O., Jones G. E. 1976; A procedure for specifically selecting temperature sensitive mutants of tobacco mosaic virus. Molecular and General Genetics 14s:307–309
    [Google Scholar]
  10. Dawson W. O., White J. L. 1978; Characterisation of a temperature sensitive mutant of tobacco mosaic virus deficient in synthesis of all RNA species. Virology 90:209–213
    [Google Scholar]
  11. Dawson W. O., White J. L. 1979; A temperature sensitive mutant of tobacco mosaic virus deficient in synthesis of single-stranded RNA. Virology 93:104–110
    [Google Scholar]
  12. De Jaoer C. P. 1976; Genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment tests. Virology 70:151–163
    [Google Scholar]
  13. De Jager C. P. 1978; Genetische analyse van cowpea-mozatekvirusmutanten. Thesis Landbouwhogeschool Wageningen;
    [Google Scholar]
  14. De Jager C. P., Breekland L. 1979; Evidence for intrastrand complementation in cowpea mosaic virus infection. Virology 99: (in the press)
    [Google Scholar]
  15. Dingjan-Versteegh A., Van Vloten-Doting L., Jaspars E. M. J. 1972; Alfalfa mosaic virus hybrids constructed by exchanging nucleoprotein components. Virology 49:716–722
    [Google Scholar]
  16. Eckhart W. 1977; Genetics of polyoma virus and simian virus 40. In Comprehensive Virology vol 9 pp 1–26 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  17. Gierer A., Mundry K. W. 1958; Production of mutants of tobacco mosaic virus by chemical alteration of its ribonucleic acid in vitro. Nature, London 182:1457–1458
    [Google Scholar]
  18. Habili N., Francki R. I. B. 1974; Comparative studies on tomato aspermy and cucumber mosaic viruses. III. Further studies on relationship and construction of a virus from parts of the two viral genomes. Virology 61:443–449
    [Google Scholar]
  19. Hartmann D., Mohier E., Leroy C., Hirth L. 1976; Genetic analysis of alfalfa mosaic virus. Virology 74:470–480
    [Google Scholar]
  20. Heijtinik R. A., Houwing C. J., Jaspars E. M. J. 1977; Molecular weights of particles and RNA’s of alfalfa mosaic virus. Number of subunits in protein capsids. Biochemistry 16:4684–4693
    [Google Scholar]
  21. Hull R. 1969; Alfalfa mosaic virus. Advances in Virus Research 15:365–433
    [Google Scholar]
  22. Jamieson A. T., Subak-Sharpe J. H. 1974; Biochemical studies on the Herpes simplex virus-specified deoxycytidine kinase activity. Journal of General Virology 24:481–492
    [Google Scholar]
  23. Kado C. L., Knight C. A. 1966; Location of a local lesion gene in tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences of the United States of America 55:1276–1283
    [Google Scholar]
  24. Kraal B. 1975; Amino acid analysis of alfalfa mosaic virus coat proteins: an aid for viral strain identification. Virology 66:336–340
    [Google Scholar]
  25. Kruseman J., Kraal B., Jaspars E. M. J., Bol J. F., Brederode F. Th., Veldstra H. 1971; Molecular weight of the coat protein of alfalfa mosaic virus. Biochemistry 10:447–455
    [Google Scholar]
  26. Lane L. 1974; The bromoviruses. Advances in Virus Research 19:151–220
    [Google Scholar]
  27. Linthorst H. J. M., Bol J. F., Jaspars E. M. J. 1980; Lack of temperature sensitivity of RNA-dependent RNA polymerases isolated from tobacco plants infected with ts mutants of alfalfa mosaic virus. Journal of General Virology 46:000
    [Google Scholar]
  28. Marchoux G., Marrou J., Quiot J. B. 1974; Complementation entre ARN de differentes souches du virus de la mosalque du concombre. Mise en evidence d’une interaction entre deux ARN pour determiner un type de symptome. Comptes rendus hebdomadaires des seances de I’Academie des sciences, Serie D 279:1943–1946
    [Google Scholar]
  29. Mohier E., Hirth L., Le Meur M. A., Gerlinger P. 1975; Translation of alfalfa mosaic virus RNA’s in mammalian cell-free systems. Virology 68:349–359
    [Google Scholar]
  30. Mohier E., Hirth L., Le Meur M. A., Gerlinger P. 1976; Analysis of alfalfa mosaic virus 17S RNA translational products. Virology 71:615–618
    [Google Scholar]
  31. Nishiguchi M., Motoyoshi F., Oshima N. 1978; Behaviour of a temperature sensitive strain of tobacco mosaic virus in tomato leaves and protoplasts. Journal of General Virology 39:53–61
    [Google Scholar]
  32. Pinck L., Fauquet C. 1975; Analysis of the pancreatic-ribonuclease digestion products of alfalfa mosaic virus ribonucleic acid: sequence homologies between the different RNAs. European Journal of Biochemistry 57:441–451
    [Google Scholar]
  33. Robinson D. J. 1973; Properties of two temperature-sensitive mutants of tobacco rattle virus. Journal of General Virology 21:499–506
    [Google Scholar]
  34. Robinson D. J. 1977; A variant of tobacco rattle: evidence for a second gene in RNA-2. Journal of General Virology 35:37–43
    [Google Scholar]
  35. Rutoers A. S. 1977; In vitro and in vivo translation of the RNAs of alfalfa mosaic virus. Thesis University of Leiden;
    [Google Scholar]
  36. Singer B., Fraenkel-Conrat H. 1967; Chemical modification of viral RNA. VI. The action of V-methyl-V′-nitro-TV-nitrosoguanidine. Proceedings of the National Academy of Sciences of the United States of America 58:234–239
    [Google Scholar]
  37. Spandidos D. A., Graham A. F. 1975a; Complementation of defective reovirus by ts mutants. Journal of Virology 15:954–963
    [Google Scholar]
  38. Spandidos D. A., Graham A. F. 1975b; Complementation between temperature-sensitive and deletion mutants of reovirus. Journal of Virology 16:1444–1452
    [Google Scholar]
  39. Spoerel N., Herrlich P., Bicle T. 1979; A novel bacteriophage defence mechanism: the anti-restriction protein. Nature, London 278:30–34
    [Google Scholar]
  40. Van Tol R. A. L., Van Vloten-Doting L. 1979; Translation of alfalfa mosaic virus RNA 1 in the mRNA-dependent translation system from rabbit reticulocyte lysates. European Journal of Biochemistry 93:461–468
    [Google Scholar]
  41. Van Vloten-Doting L. 1976; Similarities and differences between viruses with a tripartite genome. Annates de l’Institute Pasteur 127A:119–129
    [Google Scholar]
  42. Van Vloten-Doting L., Jaspars E. M. J. 1967; Enhancement of infectivity by combination of two ribonucleic acid components from alfalfa mosaic virus. Virology 33:684–693
    [Google Scholar]
  43. Van Vloten-Doting L., Jaspars E. M. J. 1972; The uncoating of alfalfa mosaic virus by its own RNA. Virology 48:699–708
    [Google Scholar]
  44. Van Vloten-Doting L., Kruseman J., Jaspars E. M. J. 1968; The biological function and mutual dependence of bottom component and top component a of alfalfa mosaic virus. Virology 34:728–737
    [Google Scholar]
  45. Williams J. F., Young C. S. H., Austin P. E. 1974; Genetic analysis of human adenovirus type 5 in permissive and nonpermissive cells. Cold Spring Harbor Symposia on Quantitative Biology 39:427–437
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-46-2-415
Loading
/content/journal/jgv/10.1099/0022-1317-46-2-415
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error