@article{mbs:/content/journal/jgv/10.1099/0022-1317-46-2-381, author = "Fritsch, Christiane and Mayo, M. A. and Murant, A. F.", title = "Translation Products of Genome and Satellite RNAs of Tomato Black Ring Virus", journal= "Journal of General Virology", year = "1980", volume = "46", number = "2", pages = "381-389", doi = "https://doi.org/10.1099/0022-1317-46-2-381", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-46-2-381", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY In wheat germ extracts and reticulocyte lysates the genome RNA molecules of tomato black ring virus (TBRV), RNA-1 (mol. wt. 2.8 × 106) and RNA-2 (mol. wt. 1.6 × 106), were translated into products of maximum mol. wt. 2.2 × 105 and 1.6 × 105, respectively. These products represent about 80% and 100% of the coding capacity of the two RNA species. The 1.6 × 105 mol. wt. polypeptide reacted with antiserum to TBRV particles but the translation products of RNA-1 did not; this is additional evidence that RNA-2 contains the coat protein cistron. Satellite RNA molecules (RNA-3, mol. wt. 4.8 × 105) associated with strain S of TBRV, like those associated with strain G, were translated in wheat germ extracts into a polypeptide of mol. wt. 4.8 × 104; this did not react with TBRV antiserum. Protease digestion released peptides from the translation product of strain S RNA-3 which were different from those released from the translation product of strain G RNA-3, suggesting that the two kinds of satellite RNA molecules differ in base sequence.", }