Studies on the Morphogenesis of Polyhedral Inclusion Bodies of a Baculovirus NPV Free

Abstract

SUMMARY

Electron micrographs are presented which illustrate ultrastructural relationships between the polyhedron macromolecular lattice, polyhedron membrane and condensed fibrous sheets in cells infected with nuclear polyhedrosis virus. A fibrous material appears to be implicated in the production of all three structures in the infected nuclei. Fibres within an unorganized network were considered to bind together to form a matrix which became ordered into a macromolecular lattice. Accumulations of fibrous masses seen at the periphery of the polyhedron apparently condensed to form the polyhedron membrane. Single and double condensed fibrous sheets are also found in the nucleus; the ultrastructural studies indicate that they too may have arisen by condensation of the fibrous material.

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1980-02-01
2024-03-29
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References

  1. Bergold O. H. 1963; The nature of nuclear polyhedrosis viruses. In Insect Pathology vol 1 pp 413–456 Edited by Steinhaus E. A. New York: Academic Press;
    [Google Scholar]
  2. Brown M., Crawford A. M., Faulkner P. 1979; Genetic analysis of a baculovirus, Autographa californica nuclear polyhedrosis virus. I. Isolation of temperature sensitive mutants and assortment into complementation groups. Journal of Virology 31:190–198
    [Google Scholar]
  3. Falcon L. A., Hess R. T. 1977; Electron microscope study on the replication of Autographa nuclear polyhedrosis virus and Spodoptera nuclear polyhedrosis virus in Spodoptera exigua. Journal of Invertebrate Pathology 29:36–43
    [Google Scholar]
  4. Gardiner G. R., Stockdale H. 1975; Two tissue culture media for production of lepidopteran cells and nuclear polyhedrosis viruses. Journal of Invertebrate Pathology 25:363–370
    [Google Scholar]
  5. Harrap K. A. 1972; The structure of nuclear polyhedrosis virsues. I. The inclusion body. Virology 50:114–123
    [Google Scholar]
  6. Hess R. T., Falcon L. A. 1978; Electron microscope observations of the membrane surrounding polyhedral inclusion bodies of insects. Archives of Virology 56:169–176
    [Google Scholar]
  7. Hink W. F., Vail P. V. 1973; A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN–368) cell line. Journal of Invertebrate Pathology 22:168–174
    [Google Scholar]
  8. Hughes K. M. 1978; The macromolecular lattices of polyhedra. Journal of Invertebrate Pathology 31:217–224
    [Google Scholar]
  9. Mackinnon E. A., Henderson J. F., Stoltz D. B., Faulkner P. 1974; Morphogenesis of nuclear polyhedrosis virus under conditions of prolonged passage in vitro. Journal of Ultrastructural Research 49:419–435
    [Google Scholar]
  10. Morgan C., Bergold G. H., Moore D. H., Rose H. M. 1955; The macromolecular paracrystalline lattice of insect viral polyhedral bodies demonstrated in ultrathin sections examined in the electron microscope. Journal of Biophysical & Biochemical Cytology 1:187–190
    [Google Scholar]
  11. Potter K. N., Faulkner P., Mackinnon E. A. 1976; Strain selection during serial passage of Trichoplusia ni nuclear polyhedrosis virus. Journal of Virology 18:1040–1050
    [Google Scholar]
  12. Rohrmann G. F. 1977; Characterization of N–polyhedrin of two baculovirus strains pathogenic for Orgyia pseudotsugata. Biochemistry 16:1630–1634
    [Google Scholar]
  13. Summers M. D. 1975; Biophysical and biochemical properties of baculoviruses. In Baculoviruses for Insect Pest Control: Safety Considerations pp 17–29 Edited by Summers M. D., Engler R., Falcon L. A., Vail P. Washington, D,. C.: American Society for Microbiology;
    [Google Scholar]
  14. Summers M. D., Arnott H. J. 1969; Ultrastructural studies on inclusion formation and virus occlusion in nuclear polyhedrosis and granulosis virus–infected cells of Trichoplusia ni (Hiibney). Journal of Ultrastructural Research 28:462–480
    [Google Scholar]
  15. Summers M. D., Smith G. E. 1975; Comparative studies of baculovirus granulins and polyhedrins. Intervirology 6:168–180
    [Google Scholar]
  16. Vaughn J. L., Goodwin R. H., Tompkins G. J., Mccawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13:213–217
    [Google Scholar]
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