Electron micrographs are presented which illustrate ultrastructural relationships between the polyhedron macromolecular lattice, polyhedron membrane and condensed fibrous sheets in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus. A fibrous material appears to be implicated in the production of all three structures in the infected nuclei. Fibres within an unorganized network were considered to bind together to form a matrix which became ordered into a macromolecular lattice. Accumulations of fibrous masses seen at the periphery of the polyhedron apparently condensed to form the polyhedron membrane. Single and double condensed fibrous sheets are also found in the nucleus; the ultrastructural studies indicate that they too may have arisen by condensation of the fibrous material.
BrownM.,
CrawfordA. M.,
FaulknerP.1979; Genetic analysis of a baculovirus, Autographa californica nuclear polyhedrosis virus. I. Isolation of temperature sensitive mutants and assortment into complementation groups. Journal of Virology 31:190–198
FalconL. A.,
HessR. T.1977; Electron microscope study on the replication of Autographa nuclear polyhedrosis virus and Spodoptera nuclear polyhedrosis virus in Spodoptera exigua. Journal of Invertebrate Pathology 29:36–43
GardinerG. R.,
StockdaleH.1975; Two tissue culture media for production of lepidopteran cells and nuclear polyhedrosis viruses. Journal of Invertebrate Pathology 25:363–370
HessR. T.,
FalconL. A.1978; Electron microscope observations of the membrane surrounding polyhedral inclusion bodies of insects. Archives of Virology 56:169–176
HinkW. F.,
VailP. V.1973; A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN–368) cell line. Journal of Invertebrate Pathology 22:168–174
MackinnonE. A.,
HendersonJ. F.,
StoltzD. B.,
FaulknerP.1974; Morphogenesis of nuclear polyhedrosis virus under conditions of prolonged passage in vitro. Journal of Ultrastructural Research 49:419–435
MorganC.,
BergoldG. H.,
MooreD. H.,
RoseH. M.1955; The macromolecular paracrystalline lattice of insect viral polyhedral bodies demonstrated in ultrathin sections examined in the electron microscope. Journal of Biophysical & Biochemical Cytology 1:187–190
PotterK. N.,
FaulknerP.,
MackinnonE. A.1976; Strain selection during serial passage of Trichoplusia ni nuclear polyhedrosis virus. Journal of Virology 18:1040–1050
SummersM. D.1975; Biophysical and biochemical properties of baculoviruses. In Baculoviruses for Insect Pest Control: Safety Considerations pp 17–29 Edited by
SummersM. D.,
EnglerR.,
FalconL. A.,
VailP.
Washington, D,. C.: American Society for Microbiology;
SummersM. D.,
ArnottH. J.1969; Ultrastructural studies on inclusion formation and virus occlusion in nuclear polyhedrosis and granulosis virus–infected cells of Trichoplusia ni (Hiibney). Journal of Ultrastructural Research 28:462–480
VaughnJ. L.,
GoodwinR. H.,
TompkinsG. J.,
MccawleyP.1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13:213–217