Peritoneal macrophages from mice infected with herpes simplex virus type 2 (HSV-2) exhibited extrinsic antiviral resistance. When the macrophages were cocultivated in vitro with virus-infected cells the yield of virus was reduced markedly. Activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after HSV-2 infection. The extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. The macrophage antiviral activity was also dose-dependent, with approx. 106 macrophages (macrophage:host cell ratio of approx. 2:1) reducing virus plaques by > 90% and virus yield 1.5 to 3.0 log10. Comparable extrinsic antiviral activity was also exhibited by Corynebacterium parvum- or thioglycollate-elicited peritoneal macrophages. The macrophage activity was not species-specific, activity on Vero cells or syngeneic mouse embryo fibroblasts being comparable. Activity was also not virus-specific, as the active macrophages also inhibited vesicular stomatitis virus (VSV). The antiviral effects required viable macrophages; cell lysates did not inhibit virus growth.
BiancoC.,
EdelsonP. J.1978; Plasma membrane expression of macrophage differentiation. In Molecular Basis of Cell-Cell Interaction (Birth Defects vol 14 no. 2 pp 119–124 Edited by
LernerR. A.
New York: Alan R. Liss;
BlandenR. V.1971; Mechanisms of recovery from a generalized viral infection: mousepox. II. Passive transfer of recovery mechanisms with immune lymphoid cells. Journal of Experimental Medicine 133:1074–1089
BrierA. M.,
SnydermanR.,
MergenhagenS. E.,
NotkinsA. L.1970; Inflammation and herpes simplex virus: release of a chemotaxis-generating factor from infected cells. Science 170:1104–1106
DesmyterJ.,
MelnickJ. L.,
RawlsW. E.1968; Defectiveness of interferon production and of rubella virus interference in a line of African green monkey kidney cells (Vero). Journal of Virology 2:955–961
HandwergerB. S.,
KorenH. S.1976; The nature of the effector cell in antibody dependent, cell-mediated cytolysis (ADCC): the cytotoxic activity of murine tumor cells and peritoneal macrophages. Clinical Immunology and Immunopathology 5:272–281
HibbsJ. B.,
LambertL. H.,
RemingtonJ. S.1972; Possible role of macrophage mediated nonspecific cytotoxicity in tumor resistance. Nature New Biology 235:48–50
JohnsonR. T.1964; The pathogenesis of herpesvirus encephalitis. II. A cellular basis for the development of resistance with age. Journal of Experimental Medicine 120:359–374
KirchnerH.,
ScottM. T.,
HirtH. M.,
MunkK.1978; Protection of mice against viral infection by Corynebacterium parvum and Bordetella pertussis. Journal of General Virology 41:97–104
MacfarlanR. I.,
BurnsW. H.,
WhiteD. O.1977; Two cytotoxic cells in peritoneal cavity of virus infected mice: antibody dependent macrophages and nonspecific killer cells. Journal of Immunology 119:1569–1574
McfarlandH. F.,
JohnsonR. T.1975; The role of the inflammatory response in viral infections. In Viral Immunology and Immunopathology pp 137–148 Edited by
NotkinsA. L.
New York: Academic Press;
McgeorgeM. B.,
MorahanP. S.1978; Comparison of various macrophage-inhibitory agents on vaginal and systemic herpes simplex virus type 2 infections. Infection and Immunity 22:623–626
MorahanP. S.,
GrossbergS. E.1970; Age-related cellular resistance of the chicken embryo to viral infections. I. Interferon and natural resistance to myxoviruses and vesicular stomatitis virus. Journal of Infectious Diseases 121:615–623
MorahanP. S.,
BreinigM. C.,
McgeorgeM. B.1977a; Immune responses to vaginal or systemic infection of BALB/c mice with herpes simplex virus type 2. Journal of Immunology 119:2030–2036
MorahanP. S.,
GlasgowL. A.,
CraneJ. L.,
KernE. R.1977b; Comparison of antiviral and antitumor activity of activated macrophages. Cellular Immunology 18:404–415
MorahanP. S.,
KernE. A.,
GlasgowL. A.1977c; Immunomodulator induced resistance against herpes simplex virus. Proceedings of the Society for Experimental Biology and Medicine 154:615–620
NathanC. F.,
AsofskyR.,
TerryW. D.1977; Characterization of the nonphagocytic adherent cell from the peritoneal cavity of normal and BCG treated mice. Journal of Immunology 188:1612–1621
SchleupnerC. J.,
OlsenG. A.,
GlasgowL. A.1979; Activation of reticuloendothelial cells following infection with murine cytomegalovirus. Journal of Infectious Diseases 139:641–652
SilversteinS. C.1975; The role of mononuclear phagocytes in viral immunity. In Mononuclear Phagocytes in Immunity, Infection and Pathology pp 557–573 Edited by
Van FurthR.
Oxford: Blackwell Scientific Publishers;
StevensJ. G.,
CookM. L.1971; Restriction of herpes simplex virus by macrophages. An analysis of the cell-virus interaction. Journal of Experimental Medicine 133:19–38
WrightL. B.,
MorahanP. S.1976; Macrophage activity against herpes simplex virus, type 2, in vivo and in vitro. Virginia Journal of Science 27:93 (abstract)
ZismanB.,
HirschM. S.,
AllisonA. C.1970; Selective effects of antimacrophage serum, silica and antilymphocyte serum on the pathogenesis of herpesvirus infection in young adult mice. Journal of Immunology 104:1155–1159