@article{mbs:/content/journal/jgv/10.1099/0022-1317-46-2-291, author = "Morahan, Page S. and Morse, Stephen S. and McGeorge, Margaret B.", title = "Macrophage Extrinsic Antiviral Activity during Herpes Simplex Virus Infection", journal= "Journal of General Virology", year = "1980", volume = "46", number = "2", pages = "291-300", doi = "https://doi.org/10.1099/0022-1317-46-2-291", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-46-2-291", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Peritoneal macrophages from mice infected with herpes simplex virus type 2 (HSV-2) exhibited extrinsic antiviral resistance. When the macrophages were cocultivated in vitro with virus-infected cells the yield of virus was reduced markedly. Activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after HSV-2 infection. The extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. The macrophage antiviral activity was also dose-dependent, with approx. 106 macrophages (macrophage:host cell ratio of approx. 2:1) reducing virus plaques by > 90% and virus yield 1.5 to 3.0 log10. Comparable extrinsic antiviral activity was also exhibited by Corynebacterium parvum- or thioglycollate-elicited peritoneal macrophages. The macrophage activity was not species-specific, activity on Vero cells or syngeneic mouse embryo fibroblasts being comparable. Activity was also not virus-specific, as the active macrophages also inhibited vesicular stomatitis virus (VSV). The antiviral effects required viable macrophages; cell lysates did not inhibit virus growth.", }