%0 Journal Article %A Schultz, M. %A Crowell, R. L. %T Acquisition of Susceptibility to Coxsackievirus A2 by the Rat L8 Cell Line During Myogenic Differentiation %D 1980 %J Journal of General Virology, %V 46 %N 1 %P 39-49 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-46-1-39 %I Microbiology Society, %X SUMMARY Coxsackievirus A2 was propagated to high titres in the established rat L8 myogenic cell line during the stage of differentiation characterized by fusion of myoblasts into multinucleated myotubes (192 h post-plating cultures). In contrast, pre-fusion mononucleated L8 cultures infected 24 h after plating were refractory to Coxsackievirus A2 infection, as was a non-fusing clonal variant of the L8 line (L8CL3-U) regardless of the age of the latter cultures. The development of virus susceptibility in the older L8 cultures correlated with a marked virus-specific c.p.e. as evidenced by vacuolated degenerating myotubes with disrupted cytoplasm, whereas no c.p.e. was seen following infection of the young L8 or variant L8CL3-U cultures. The restriction of Coxsackievirus A2 replication in young L8 and L8CL3-U cultures occurred after virus attachment. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-46-1-39