1887

Abstract

SUMMARY

The biochemical basis for variation in foot-and-mouth disease virus (FMDV) has been explored by analysis of the virus RNA and the virus-induced and structural proteins of three isolates of the virus. Two of the isolates were from serotype A and the third was from serotype O. Hybridization studies of the RNAs showed greater than 80% homology between the two type A viruses and about 65% homology between the two type A viruses and the virus of type O. The ribonuclease T maps of the three viruses gave distinct patterns typical of FMDV, but did not show that any two of the three viruses were more closely related. The virus-induced primary translation products, P88, P52 and P100 isolated from infected cells, were compared by tryptic peptide analysis. Combinations of H- and C-leucine-labelled polypeptides were hydrolysed with trypsin and resolved on an ion-exchange column. Much greater differences were found in P88 than in P52 or P100, indicating that the major variation occurs in the region of the genome coding for the structural proteins. Similar analysis of combinations of the structural proteins of the three viruses showed that there were differences in VP1, VP2 and VP3 and these results were supported by those obtained by PAGE analysis of the V8 protease cleavage products.

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1980-01-01
2022-06-29
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References

  1. Adam K. H., Strohmaier K. 1974; Isolation of the coat proteins of foot-and-mouth disease virus and analysis of the composition and IV-terminal end groups. Biochemical and Biophysical Research Communications 18:185–192
    [Google Scholar]
  2. Bachrach H. L., Swaney J. B., Vande Woude G. F. 1973; Isolation of the structural polypeptides of foot-and-mouth disease virus and analysis of their C terminal sequences. Virology 52:520–528
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Brown F., Smale C. J. 1970; Demonstration of three specific sites on the surface of foot-and-mouth disease virus by antibody complexing. Journal of General Virology 7:115–127
    [Google Scholar]
  5. Burrell C. J., Cooper P. D. 1973; A-terminal aspartate, glycine and serine in poliovirus capsid proteins. Journal of General Virology 21:443–451
    [Google Scholar]
  6. Burroughs J. N., Rowlands D. J., Sangar D. V., Talbot P., Brown F. 1971; Further evidence for multiple proteins in the foot-and-mouth disease virus particle. Journal of General Virology 13:73–84
    [Google Scholar]
  7. Burroughs J. N., Doel T. R., Brown F. 1978; Relationship of San Miguel sea lion virus to other members of the calicivirus group. Intervirology 10:51–59
    [Google Scholar]
  8. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  9. Cooper P. D., Stancek D., Summers D. F. 1970; Synthesis of double-stranded RNA by poliovirus temperature-sensitive mutants. Virology 40:971–977
    [Google Scholar]
  10. Cords C. E., Holland J. J. 1964; Interference between enteroviruses and conditions effecting its reversal. Virology 22:226–234
    [Google Scholar]
  11. Darby G., Minson A. C. 1973; The structure of tobacco rattle virus ribonucleic acids: common nucleotide sequences in the RNA species. Journal of General Virology 21:285–295
    [Google Scholar]
  12. De Wachter R., Fiers W. 1972; Preparative two-dimensional polyacrylamide electrophoresis of [S2P] labelled RNA. Analytical Biochemistry 49:184–197
    [Google Scholar]
  13. Doel T. R., Brown F. 1978; Tryptic peptide analysis of the structural proteins of vesicular stomatitis virus. Journal of General Virology 38:351–361
    [Google Scholar]
  14. Doel T. R., Sangar D. V., Rowlands D. J., Brown F. 1978; A re-appraisal of the biochemical map of foot-and-mouth disease virus RNA. Journal of General Virology 41:395–404
    [Google Scholar]
  15. Eisenthal R., Cornish-Bowden A. 1974; The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochemical Journal 139:715–720
    [Google Scholar]
  16. Flanegan J. B., Baltimore D. 1979; Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide. Journal of Virology 29:352–360
    [Google Scholar]
  17. Frisby D. P., Newton C., Carey N. H., Fellner P., Newman J. F. E., Harpis T. J. R., Brown F. 1976; Oligonucleotide mapping of picornavirus RNAs by two-dimensional electrophoresis. Virology 71:379–388
    [Google Scholar]
  18. Harris T. J. R., Brown F. 1975; Correlation of polypeptide composition with antigenic variation in swine vesicular disease and Coxsackie B5 viruses. Nature, London 258:758–760
    [Google Scholar]
  19. Harris T. J. R., Brown F. 1977; Biochemical analysis of a virulent and an avirulent strain of foot-and-mouth disease virus. Journal of General Virology 34:87–105
    [Google Scholar]
  20. Korant B. D. 1979; Role of cellular and viral proteases in the processing of picornavirus proteins. In The Molecular Biology of the Picornaviruses pp 149–173 Edited by Perez-Bercoff R. New York: Plenum Press;
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. Laporte J. 1971; Workshop on foot-and-mouth disease virus. p 147 Proceedings of the Second International Congress for Virology Budapest:
    [Google Scholar]
  23. Laskey R. A., Mills A. D. 1977; Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Letters 82:314–316
    [Google Scholar]
  24. Lundquist R. E., Ehrenfeld E., Maizel J. V. 1974; Isolation of a viral polypeptide associated with poliovirus replication complex. Proceedings of the National Academy of Sciences of the United States of America 71:4773–4777
    [Google Scholar]
  25. Newman J. F. E., Cartwright B., Doel T. R., Brown F. 1979; Purification and identification of the RNA dependent RNA polymerase of foot-and-mouth disease virus. Journal of General Virology 45:497–507
    [Google Scholar]
  26. O’farrell P. Z. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  27. Robson K. J. H., Harpis T. J. R., Brown F. 1977; An assessment by competition hybridization of the sequence homology between the RNAs of the seven serotypes of FMDV. Journal of General Virology 37:271–276
    [Google Scholar]
  28. Robson K. J. H., Crowther J. R., King A. M. Q., Brown F. 1979; Comparative biochemical and serological analysis of five isolates of a single serotype of foot-and-mouth disease virus. Journal of General Virology 45:579–590
    [Google Scholar]
  29. Sangar D. V., Rowlands D. J., Cavanagh D., Brown F. 1976; Characterization of the minor polypeptides in the foot-and-mouth disease virus particle. Journal of General Virology 31:35–46
    [Google Scholar]
  30. Sangar D. V., Black D. N., Rowlands D. J., Brown F. 1977; Biochemical mapping of the foot-and-mouth disease virus genome. Journal of General Virology 35:281–297
    [Google Scholar]
  31. Strohmaier K., Wittman-Liebold B., Geisser A.-W. 1978; The N-terminal sequence of three coat proteins of foot-and-mouth disease virus. Biochemical and Biophysical Research Communications 85:1640–1645
    [Google Scholar]
  32. Talbot P., Brown F. 1972; A model for foot-and-mouth disease virus. Journal of General Virology 15:163–170
    [Google Scholar]
  33. Talbot P., Rowlands D. J., Burroughs J. N., Sangar D. V., Brown F. 1973; Evidence for a group protein in foot-and-mouth disease virus particles. Journal of General Virology 19:369–380
    [Google Scholar]
  34. Wecker E., Lederhilger G. 1964; Genomic masking produced by double infection of HeLa cells with heterotypic polioviruses. Proceedings of the National Academy of Sciences of the United States of America 52:705–709
    [Google Scholar]
  35. Ziola B. R., Scraba D. G. 1974; Structure of the Mengo virion, Part i. Polypeptide and ribonucleate components of the virus particle. Virology 57:531–542
    [Google Scholar]
  36. Ziola B. R., Scraba D. G. 1976; Structure of the Mengo virion, Part 4. Amino and carboxyl terminal analyses of the major capsid polypeptides. Virology 71:111–121
    [Google Scholar]
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