1887

Abstract

SUMMARY

The polypeptide patterns of five strains of mumps virus have been compared under uniform electrophoresis conditions. These strains included the MJ and RW strains, recently isolated from human cerebrospinal fluid, the neuro-adapted Kilham strain and the chick embryo-adapted Enders and Jeryl Lynn strains. A similar pattern of polypeptides, featuring six common and virus-specific species, was obtained with purified virus grown , in chick embryo fibroblasts (CEF) or in Vero cells. These polypeptides have been designated VP1 (mol. wt. 200000), VP2 (mol. wt. 80000 or 75000), VP3 (mol. wt. 68000), VP4 (mol. wt. 58000), VP5 (mol. wt. 45000), VP6 (mol. wt. 39000). The virus glycoproteins were identified as VP2 and VP4; the nucleocapsid-associated proteins as VP1, VP3 and VP5. Additional polypeptides occurring both in uninfected Vero cells and in uninfected CEFs appeared in polypeptide profiles of purified mumps virus preparations, and could not be designated virus-specific polypeptides. Although strain differences were minimal, we did observe (1) an extra protein, nucleocapsid-associated, in patterns from the chick-adapted Enders strain of mumps virus and (2) strain-dependent variability in the size of VP2.

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1980-01-01
2021-10-27
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References

  1. Blair C. D., Duesberg P. H. 1970; Myxovirus nucleic acid. Annual Review of Microbiology 24:539–574
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Buetti E., Choppin P. W. 1977; The transcriptase complex of the paramyxovirus SV5. Virology 82:493–508
    [Google Scholar]
  4. Buynak E. B., Hilleman M. R. 1966; Live attenuated mumps virus vaccine, 1. Vaccine development. Proceedings of the Society for Experimental Biology and Medicine 123:768–775
    [Google Scholar]
  5. Caliguiri L. A., Klenk H. D., Choppin P. 1969; The proteins of the parainfluenza virus SV5.1. Separation of virion polypeptides by polyacrylamide gel electrophoresis. Virology 39:460–466
    [Google Scholar]
  6. Enders J. F., Levens J. H., Stokes J. Jun, Maris E. P., Berenberg W. 1946; Attenuation of virulence with retention of antigenicity of mumps virus after passage in the embryonated egg. Journal of Immunology 54:283–291
    [Google Scholar]
  7. Hall W. W., Martin S. J. 1973; Purification and characterization of measles virus. Journal of General Virology 19:175–188
    [Google Scholar]
  8. Hewitt J. A., Nermut M. V. 1977; A morphological study of the m-protein of Sendai virus. Journal of General Virology 34:127–136
    [Google Scholar]
  9. Hightower L. E., Morrison T. G., Bratt M. A. 1975; Relationships among the polypeptides of Newcastle disease virus. Journal of Virology 16:1599–1607
    [Google Scholar]
  10. Huppertz H. I., Hall W. W., Ter Meulen V. 1977; Polypeptide composition of mumps virus. Medical Microbiology and Immunology 163:251–259
    [Google Scholar]
  11. Jensik S. C., Silver S. 1976; Polypeptides of mumps virus. Journal of Virology 17:363–373
    [Google Scholar]
  12. Kilham L., Overman J. R. 1953; Natural pathogenicity of mumps virus for suckling hamsters on intracerebral inoculation. Journal of Immunology 70:147–151
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of the structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Maizel J. V. 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:179–246
    [Google Scholar]
  15. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. 1970; Nucleocapsid protein subunits of Simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 6:677–684
    [Google Scholar]
  16. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses: a comparison of Simian virus 5, Newcastle disease virus, and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  17. Nagai Y., Klenk H. D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  18. Orvell C. 1978a; Immunological properties of purified mumps virus glycoproteins. Journal of General Virology 41:517–526
    [Google Scholar]
  19. Orvell C. 1978b; Structural polypeptides of mumps virus. Journal of General Virology 41:527–540
    [Google Scholar]
  20. Peluso R. W., Lamb R. A., Choppin P. W. 1977; Polypeptide synthesis in Simian virus 5-infected cells. Journal of Virology 23:177–187
    [Google Scholar]
  21. Scheid A., Choppin P. W. 1973; Isolation and purification of the envelope proteins of Newcastle disease virus. Journal of Virology n:263–271
    [Google Scholar]
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