1887

Abstract

SUMMARY

Inducers of the Epstein-Barr virus (EBV) cycle, 5-iodo-2-deoxyuridine (IdUrd), phorbol myristate acetate (TPA) and sodium butyrate were tested for their ability to induce EBV-determined antigens, early antigen (EA) and virus capsid antigen (VCA), and to stimulate interferon (IF) production in a variety of EBV-carrying lymphoid cell lines. IdUrd and TPA induced IF production to various extents in the different lines, whereas sodium butyrate did not. There was no relationship between induction of the EBV cycle and production of IF; the two appear to be independent characteristics.

Superinfection with the transforming B95-8 virus substrain of EBV induced IF production, whereas superinfection with the abortively cytopathic, non-transforming P3HR-1 substrain had little or no IF-inducing effect, in spite of its highly potent effect on virus antigen (particularly EA) synthesis.

Analysis with specific antisera against IF showed that IF preparations produced by three different lymphoid cell lines in response to IdUrd treatment were composed of a mixture of the and antigenic types, with the latter forming a minor species. In contrast, no detectable interferon was present in spontaneously produced IF preparations from Namalwa cells, or after induction with B95-8 virus.

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1980-01-01
2022-01-17
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References

  1. Adams A., Lidin B., Strander H., Cantell K. 1975a; Spontaneous interferon production and Epstein-Barr virus antigen expression in human lymphoid cell lines. Journal of General Virology 28:219–223
    [Google Scholar]
  2. Adams A., Strander H., Cantell K. 1975b; Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. Journal of General Virology 28:207–217
    [Google Scholar]
  3. Aurelian L., Roizman B. 1965; Abortive infection of canine cells by herpes simplex virus. II. Alternative suppression of synthesis of interferon and viral constituents. Journal of Molecular Biology 11:539–548
    [Google Scholar]
  4. Dalens M., Adams A. 1977; Induction of Epstein-Barr virus associated early antigen in different lymphoid cell lines with ultraviolet-irradiated P3HR-1 virus. Virology 83:305–312
    [Google Scholar]
  5. Dalton B. J., Paucker K. 1979; Antigenic properties of human lymphoblastoid interferon. Infection and Immunity 23:244–248
    [Google Scholar]
  6. Havell E. A., Vilcek J. 1972; Production of high-titered interferon in cultures of human diploid cells. Antimicrobial Agents and Chemotherapy 2:476–484
    [Google Scholar]
  7. Havell E. A., Berman B., Ogburn C. A., Berg K., Paucker K., Vilcek J. 1975; TWO antigenically distinct species of human interferon. Proceedings of the National Academy of Sciences of the United States of America 72:2185–2187
    [Google Scholar]
  8. Havell E. A., Yip Y. K., Vilcek J. 1977; Characteristics of human lymphoblastoid (Namalwa) interferon. Journal of General Virology 38:51–59
    [Google Scholar]
  9. Havell E. A., Hayes T. G., Vilcek J. 1978; Synthesis of two distinct interferons by human fibroblasts. Virology 89:330–334
    [Google Scholar]
  10. Klein G., Dombos L., Gothoskar B. 1972; Sensitivity of Epstein-Barr virus (EBV) producer and nonproducer human lymphoblastoid cell lines to superinfection with EB-virus. International Journal of Cancer 10:44–57
    [Google Scholar]
  11. Klein G., Clements G., Zeuthen J., Westman A. 1976a; Somatic cell hybrids between human lymphoid lines. II. Spontaneous and induced patterns of the Epstein-Barr virus (EBV) cycle. International Journal of Cancer 17:715–724
    [Google Scholar]
  12. Klein G., Zeuthen J., Terasaki P., Billing R., Honig R., Jondal M., Westman A., Clements G. 1976b; Inducibility of the Epstein-Barr virus (EBV) cycle and surface marker properties of EBV-negative lymphoma lines and their in vitro EBV-converted sublines. International Journal of Cancer 18:639–652
    [Google Scholar]
  13. Klein G., Clements G., Zeuthen J., Westman A. 1977; Spontaneous and induced pattern of the Epstein-Barr virus (EBV) cycle in a new set of somatic cell hybrids. Cancer Letters 3:91–98
    [Google Scholar]
  14. Luka J., Kallin B., Klein C. 1979; Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate. Virology 94:228–231
    [Google Scholar]
  15. Nyormol O., Klein G., Adams A., Dombos L. 1973; Sensitivity to EBV superinfection and IUDR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. International Journal of Cancer 12:396–408
    [Google Scholar]
  16. Rocchi G., Hewetson J., Henle W., Henle G. 1973; Antigen expression and colony formation of lymphoblastoid cell lines after superinfection with Epstein-Barr virus. Journal of the National Cancer Institute 50:307–314
    [Google Scholar]
  17. Tan Y. H., Greene A. E. 1976; Subregional localization of the gene(s) governing the human interferon induced antiviral state in man. Journal of General Virology 32:153–155
    [Google Scholar]
  18. Tovey M. G., Lenoir G., Begon-Lours J. 1978; Activation of latent Epstein-Barr virus by antibody to human IgM. Nature, London 276:270–272
    [Google Scholar]
  19. Van Duuren B. L. 1976; Tumor-promoting and co-carcinogenic agents in chemical carcinogenesis. In Chemical Carcinogens, ACS Monograph 173 Edited by Searle C. E. Washington D.C.: American Chemical Society;
    [Google Scholar]
  20. Wagner R. R., Huang A. S. 1966; Inhibition of RNA and interferon synthesis in Krebs-2 cells infected with vesicular stomatitis virus. Virology 28:1–10
    [Google Scholar]
  21. Zur Hausen H., O’neill F., Freese U. K. 1978; Persisting oncogenic herpesvirus by the tumour promotor TPA. Nature, London 272:373–375
    [Google Scholar]
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