1887

Abstract

SUMMARY

Tracheal organ cultures were prepared from strains of mice with known susceptibility to the lethal effects of mouse cytomegalovirus. When organ cultures from susceptible mice were infected with this virus, characteristic c.p.e. developed and virus titres > 10 p.f.u./ml were produced 2 to 3 weeks after infection. Identically infected tracheal organ cultures from resistant mice consistently failed to develop significant virus c.p.e. and produced 10- to 100-fold lower titres of virus. When fibroblast cultures were infected with mouse cytomegalovirus, however, no consistent differences between susceptible and resistant mouse strains were observed. Since cytomegalovirus replicates in epithelial cells both and in tracheal organ culture, these results suggest that resistance may be based in part upon innate genetic susceptibility of epithelial cells to cytomegalovirus infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-3-737
1979-12-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/3/JV0450030737.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-3-737&mimeType=html&fmt=ahah

References

  1. Benyesh-Melnick M. 1969; Cytomegaloviruses. In Diagnostic Procedures for Viral and Rickettsial Infections pp 701–732 Edited by Lennette E. H., Schmidt N. J. New York: American Public Health Association;
    [Google Scholar]
  2. Boos J., Wheelock E. F. 1977; Progressive inhibition of T-cell function preceding clinical signs of cytomegalovirus infection in mice. Journal of Infectious Diseases 135:478–481
    [Google Scholar]
  3. Brody A. R., Craighead J. E. 1974; Pathogenesis of pulmonary cytomegalovirus infection in immuno-suppressed mice. Journal of Infectious Diseases 129:677–689
    [Google Scholar]
  4. Chalmer J. E., Mackenzie J. S., Stanley N. F. 1977; Resistance to murine cytomegalovirus linked to the major histocompatibility complex of the mouse. Journal of General Virology 37:107–114
    [Google Scholar]
  5. Clark H. F. 1978; Rabies viruses increase in virulence when propagated in neuroblastoma cell culture. Science 199:1072–1075
    [Google Scholar]
  6. Collier A. M., Clyde W. A. Jun, Denny F. W. 1969; Biologic effects of Mycoplasma pneumoniae and other mycoplasmas from man on hamster tracheal organ culture. Proceedings of the Society for Experimental Biology and Medicine 132:1153–1158
    [Google Scholar]
  7. Craighead J. E. 1971; Pulmonary cytomegalovirus infection in the adult. American Journal of Pathology 63:487–504
    [Google Scholar]
  8. Donnellan W. L., Chantro-Umporn S., Kidd J. M. 1966; The cytomegalic inclusion cell. Archives of Pathology 82:336–349
    [Google Scholar]
  9. Gardner M. B., Officer J. E., Parker J., Estes J. D., Rongy R. W. 1974; Induction of disseminated virulent cytomegalovirus infection by immunosuppression of naturally chronically infected wild mice. Infection and Immunity 10:966–969
    [Google Scholar]
  10. Jordan M. C. 1978; Interstitial pneumonia and subclinical infection after intranasal inoculation of murine cytomegalovirus. Infection and Immunity 21:275–280
    [Google Scholar]
  11. Knowles W. A. 1976; In vitro cultivation of human cytomegalovirus in thyroid epithelial cells. Archives of Virology 50:119–124
    [Google Scholar]
  12. Mäntyjärvi R. A., Selgrade M. J., Collier A. M., Hu S., Pagano J. S. 1977; Murine cytomegalovirus infection of epithelial cells in mouse tracheal ring organ culture. Journal of Infectious Diseases 136:444–448
    [Google Scholar]
  13. Martin A. M. Jun, Kurtz S. M. 1966; Cytomegalic inclusion disease. An electron microscopic histochemical study of the virus at necropsy. Archives of Pathology 82:27–34
    [Google Scholar]
  14. Michaelson-Fiske S., Arnoult J., Febvre H. 1975; Cytomegalovirus infection of human lung epithelial cells in vitro. Intervirology 5:354–363
    [Google Scholar]
  15. Naraqi S., Jackson G. G., Jonasson O., Yamashiroya H. M. 1977; Prospective study of prevalence, incidence and source of herpesvirus infections in patients with renal allografts. Journal of Infectious Diseases 136:531–540
    [Google Scholar]
  16. Neiman P. E., Reeves W., Ray G., Flournoy N., Lerner K. G., Sale G. E., Thomas E. D. 1977; A prospective analysis of interstitial pneumonia and opportunistic viral infection among recipients of allogeneic bone marrow grafts. Journal of Infectious Diseases 136:754–767
    [Google Scholar]
  17. Osborn J. E., Walker D. L. 1971; Virulence and attenuation of murine cytomegalovirus. Infection and Immunity 3:228–236
    [Google Scholar]
  18. Pollard R. B., Rand K. H., Arvin A. M., Merigan T. C. 1978; Cell-mediated immunity to cytomegalovirus infection in normal subjects and cardiac transplant patients. Journal of Infectious Diseases 137:541–549
    [Google Scholar]
  19. Rola-Pleszczynski M., Frenkel L. D., Fuccillo D. A., Henson S. A., Vincent M. M., Reynolds D. W., Stagno S., Bellanti J. A. 1977; Specific impairment of cell mediated immunity in mothers of infants with congenital infection due to CMV. Journal of Infectious Diseases 135:386–391
    [Google Scholar]
  20. Rowe W. P., Hartley J. W., Waterman S., Turner H. C., Huebner R. J. 1956; Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proceedings of the Society for Experimental Biology and Medicine 92:418–424
    [Google Scholar]
  21. St Jeor S., Rapp F. 1973; Cytomegalovirus: conversion of nonpermissive cells to a permissive state for virus replication. Science 18:1060–1061
    [Google Scholar]
  22. Selgrade M. K., Osborn J. E. 1974; Role of macrophages in resistance to murine cytomegalovirus. Infection and Immunity 10:1383–1390
    [Google Scholar]
  23. Starr S. E., Allison A. C. 1977; Role of T-lymphocytes in recovery from murine cytomegalovirus infection. Infection and Immunity 17:458–462
    [Google Scholar]
  24. Stern H., Lambert H. P., Shakespeare W. G. 1963; Isolation of cytomegalovirus in an infant with angiosarcoma. Archives of Diseases in Childhood 38:626–631
    [Google Scholar]
  25. Vesterinen E., Leinikki P., Saksela E. 1975; Cytopathogenicity of cytomegalovirus to human ecto- and endo-cervical epithelial cells in vitro. Acta Cytologica 19:473–481
    [Google Scholar]
  26. Vonka V., Anisimova E., Macek M. 1976; Replication of cytomegalovirus in human epithelioid diploid cell line. Archives of Virology 52:283–296
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-3-737
Loading
/content/journal/jgv/10.1099/0022-1317-45-3-737
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error