1887

Abstract

SUMMARY

Bone marrow of leukaemic patients, non-leukaemic patients and normal individuals were co-cultivated with the canine cell line A7573. These co-cultures were screened for retrovirus antigens by means of the indirect cytoplasmic immuno-fluorescence assay (IFA). Rabbit antisera directed against the major structural protein (P30) of woolly monkey (simian) sarcoma leukaemia virus (grown in human lymphoid cells) and Rauscher murine leukaemia virus were used for testing.

After 2 months in culture, 6 of 17 co-cultures containing cells from leukaemic patients showed positive staining in the IFA with the anti-simian virus serum. In control dog cells fluorescence was never observed. Five of the six positive cultures were derived from leukaemic children. One of 12 co-cultures of the non-leukaemic group and one of nine normal bone marrow co-cultures were positive with the simian virus antiserum. None of the 38 co-cultures stained positive in the IFA with Rauscher virus antiserum.

Absorption of the simian virus antiserum with calf serum or mouse mammary tumour virus had no dramatic effect in the IFA on positive control cells or on cells of a positive co-culture. However, absorption with purified simian virus (grown in rat cells) completely abolished these fluorescence reactions. The results provide evidence that simian sarcoma-leukaemia virus related information was present in the original bone marrow samples and that co-cultivation with permissive mammalian cells enabled the detection of virus footprints.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-3-711
1979-12-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/3/JV0450030711.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-3-711&mimeType=html&fmt=ahah

References

  1. Barendsen G. W., Janse H. C., Deys B. F., Hollander C. F. 1977; Comparison of growth characteristics of experimental tumours and derived cell cultures. Cell and Tissue Kinetics 10:469–475
    [Google Scholar]
  2. Calafat J., Hageman P. C., Ressang A. 1976; Virus-like particles in bovine sera for tissue culture. Journal of General Virology 33:151–154
    [Google Scholar]
  3. Chan E., Ohno T., Peters W. P., Kufe D. W., Sweet R. W., Spiegelman S., Gallo R. C., Gallagher R. E. 1976; Characterization of a virus (HL23V) isolated from cultured acute myelogenous leukaemic cells. Nature, London 260:266–268
    [Google Scholar]
  4. Charman H. P., Gilden R. V. 1978; Validation of radioimmunoassays for retrovirus. In Advances in Comparative Leukemia Research pp 46–49 Edited by Bentvelzen P., Hilgers J., Yohn D. Amsterdam: Elsevier;
    [Google Scholar]
  5. Chen T. R. 1977; In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Experimental Cell Research 104:255–262
    [Google Scholar]
  6. Gabelman N., Waxman S., Smith W., Douglas S. D. 1975; Appearance of C-type virus like particles after cocultivation of a human tumor cell line with rat (XC) cells. International Journal of Cancer 16:355–369
    [Google Scholar]
  7. Gallagher R. E., Gallo R. C. 1975; Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells. Science 187:350–353
    [Google Scholar]
  8. Gallagher R. E. 1977; Molecular probes for components of type C viruses in human leukemia. In Recent Advances in Cancer Research: Cell Biology, Molecular Biology, and Tumor Virology vol 2 pp 137–165 Edited by Gallo R. C. Cleveland: CRC Press;
    [Google Scholar]
  9. Giard D. J., Aaronson S. A., Todaro G. J., Kersey J. H., Dosik H., Parks W. P. 1973; In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. Journal of the National Cancer Institute 51:1417–1423
    [Google Scholar]
  10. Green R. W., Bolognesi D. P. 1974; Isolation of proteins by gel filtration in 6 m guanidinium chloride: application to RNA tumor viruses. Analytical Biochemistry 57:108–117
    [Google Scholar]
  11. Greenwood F. C., Hunter W. M., Clover J. S. 1963; The preparation of 131I-labelled human growth hormones of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  12. Haaijman J. J., Brinkhof J. A. 1977; Microfluorometric assay for immunoglobulin class and subclass levels in murine serum. Journal of Immunological Methods 14:213–224
    [Google Scholar]
  13. Joustra M., Lundgren H. 1970; Preparation of freeze-dried monomeric and immunochemically IgG by a rapid and reproducible technique. In Protides of the Biological Fluid p 511 Edited by H. Peeter Oxford: Pergamon Press;
    [Google Scholar]
  14. Koch C., Nooter K., Bentvelzen P., Haaijman J. 1977; Serological characterization of a putative human C-type oncornavirus by means of the Sepharose bead immunofluorescence assay. European Journal of Cancer 13:1397–1403
    [Google Scholar]
  15. Kurth R., Teich N. M., Weiss R., Oliver R. T. 1977; Natural human antibodies reactive with primate type C viral antigens. Proceedings of the National Academy of Sciences of the United States of America 74:1237–1241
    [Google Scholar]
  16. Leerhoy J. 1965; Cytopathic effect of Rubella virus in a rabbit cornea cell line. Science 149:633–634
    [Google Scholar]
  17. Lilly F., Steeves R. 1974; Antigens of murine leukemia viruses. Biochimica et Biopkysica Acta 355:105–118
    [Google Scholar]
  18. March S. C., Parikh I., Cuatrecasas P. 1974; A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Analytical Biochemistry 60:149–152
    [Google Scholar]
  19. Nelson-Rees W. A., Owens R. B., Arnstein P., Kniazeff A. J. 1976; Source, alterations, characteristics and use of a new dog cell line (Cf2Th). In Vitro 12:665–669
    [Google Scholar]
  20. Nooter K., Aarssen A. M., Bentvelzen P., De Groot F. G., Van Pelt F. G. 1975; Isolation of an infectious C-type oncornavirus from human leukaemic bone marrow cells. Nature, London 256:595–597
    [Google Scholar]
  21. Nooter K., Bentvelzen P., Zurcher C., Rhim J. 1977; Detection of human C-type ‘helper’ viruses in human leukaemic bone marrow cells with murine sarcoma virus transformed human and rat nonproducer cells. International Journal of Cancer 19:59–65
    [Google Scholar]
  22. Nooter K., Overdevest J., Dubbes R., Bentvelzen P., Coolen J. C., Zurcher C., Calafat J. 1978; Type-C oncovirus isolate from human leukemic bone marrow: further in vitro and in vivo characterization. International Journal of Cancer 21:27–34
    [Google Scholar]
  23. Okabe H., Gilden R. V., Hatanaka M., Stephenson J. R., Gallagher R. E., Gallo R. C., Tronick S. R., Aaronson S. A. 1976; Immunological and biochemical characterization of type C viruses isolated from cultured human AML cells. Nature, London 260:264–266
    [Google Scholar]
  24. Panem S., Prochownik E. V., Reale F. R., Kirsten W. H. 1975; Isolation of type-C virions from a normal fibroblast strain. Science 189:297–298
    [Google Scholar]
  25. Panem S., Ordonez N. C., Kirsten W. H., Katz A. I., Spargo B. H. 1976; C-type virus expression in systemic lupus erythematosus. New England Journal of Medicine 295:470–475
    [Google Scholar]
  26. Reitz M. S., Miller N. R., Wong-Staal F., Gallagher R. E., Gallo R. C., Gillespie D. H. 1976; Primate type-C virus nucleic acid sequences (woolly monkey and baboon types) in tissues from a patient with acute myelogenous leukemia and in viruses isolated from cultured cells of the same patient. Proceedings of the National Academy of Sciences of the United States of America 73:2113–2117
    [Google Scholar]
  27. Rhim J., Cho H., Vernon M., Arnstein P., Huebner R., Gilden R., Nelson-Rees W. 1975; Characterization of non-producer human cells induced by Kirsten sarcoma virus. International Journal of Cancer 16:840–849
    [Google Scholar]
  28. Seabright M. 1971; A rapid banding technique for human chromosomes. The Lancet ii:971–972
    [Google Scholar]
  29. Selden J. R., Moorhead P. S., Oehlert M. L., Patterson D. F. 1975; The Giemsa banding pattern of the canine karyotype. Cytogenetics and Cell Genetics 15:380–387
    [Google Scholar]
  30. Sherr C. J., Todaro G. J. 1974; Type C viral antigens in man. I. Antigens related to endogenous primate virus in human tumors. Proceedings of the National Academy of Sciences of the United States of America 71:4703–4707
    [Google Scholar]
  31. Snyder H. W. Jun, PiNCUS T., Fleissner E. 1975; Specificities of human immunoglobulins reactive with antigens in preparations of several mammalian type-C viruses. Virology 75:60–73
    [Google Scholar]
  32. Stephenson J. J., Aaronson S. A. 1976; Search for antigens and antibodies cross-reactive with type C viruses of the woolly monkey and gibbon ape in animal models and in humans. Proceedings of the National Academy of Sciences of the United States of America 73:1725–1729
    [Google Scholar]
  33. Strand M., August J. T. 1974; Type C RNA virus gene expression in human tissue. Journal of Virology 14:1584–1596
    [Google Scholar]
  34. Teich N. M., Weiss R. D., Salahuddin S. Z., Gallagher R. E., Gillespie D. H., Gallo R. C. 1975; Infective transmission and characterization of a C-type virus released by cultured human myeloid leukaemic cells. Nature, London 256:551–555
    [Google Scholar]
  35. Todaro G. J., Sherr C. J., Benveniste R. E., Lieber N. M., Melnick J. L. 1974; Type C viruses Of baboons: isolation from normal cell cultures. Cell 2:55–62
    [Google Scholar]
  36. Todaro G. J., Lieber M. M., Benveniste R. E., Sherr C. J., Gibbs C. J., Gajdusek D. C. 1975; Infectious primate type C viruses: three isolates belonging to a new subgroup from the brains of normal gibbons. Virology 67:335–357
    [Google Scholar]
  37. Weber K., Osborn M. J. 1969; The reliability of molecular weight determinations by dodecyl sulphate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  38. Wong-Staal F., Gillespie D., Gallo R. C. 1976; Proviral sequences of baboon endogenous type C RNA virus in DNA of human leukaemic tissues. Nature, London 262:190–194
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-3-711
Loading
/content/journal/jgv/10.1099/0022-1317-45-3-711
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error