1887

Abstract

SUMMARY

Poliovirus particle RNA has been considered to have little secondary structure. Specific binding of poliovirion RNA by antibodies against double-stranded RNA (dsRNA) has been confirmed and further characterized by a radioimmunoassay using protein A to precipitate the nucleic acid-antibody complex. Competitive binding studies between virion single-stranded RNA (ssRNA) and poly(I).poly(C) demonstrated that the dsRNA effectively inhibited binding of radiolabelled poliovirion RNA by the anti-dsRNA antibodies but the virion RNA was a poor competitor of radiolabelled dsRNA. This indicates that both RNAs reacted with the same species of antibodies in the sera, but avidity of the antibodies for dsRNA was greater than for the poliovirion RNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-3-665
1979-12-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/3/JV0450030665.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-3-665&mimeType=html&fmt=ahah

References

  1. Caliguiri L. A., Tamm I. 1968; Action of guanidine on the replication of poliovirus RNA. Virology 35:408–417
    [Google Scholar]
  2. Caliguiri L. A., Tamm I. 1970; The role of cytoplasmic membranes in poliovirus biosynthesis. Virology 42:10–111
    [Google Scholar]
  3. Calvet J. P., Pederson T. 1977; Secondary structure of heterogeneous nuclear RNA: two classes of double-stranded RNA in native ribonucleoprotein. Proceedings of the National Academy of Sciences of the United States of America 74:3705–3709
    [Google Scholar]
  4. Dunn J. J. 1976; RNase III cleavage of single-stranded RNA. Journal of Biological Chemistry 251:3807–3814
    [Google Scholar]
  5. Felsenfeld G., Miles H. T. 1967; The physical and chemical properties of nucleic acids. Annual Review of Biochemistry 36:407–448
    [Google Scholar]
  6. Frisby D. P., Cotter R. I., Richards B. M. 1977; Structural studies of encephalomyocarditis virus RNA both in situ and in free solution. Journal of General Virology 37:311–322
    [Google Scholar]
  7. Guigues M., Leng M. 1976; Antibodies to poly(I).poly(C): purification and interaction with polynucleotides. European Journal of Biochemistry 69:615–624
    [Google Scholar]
  8. Johnston M. I., Stollar B. D., Torrence P. F., Witkop B. 1975; Structural features of double-stranded polyribonucleotides required for immunological specificity and interferon induction. Proceedings of the National Academy of Sciences of the United States of America 72:4564–4568
    [Google Scholar]
  9. Kessler S. W. 1975; Rapid isolation of antigens from cells with a Staphylococcal protein A-antibody absorbant: parameters of the interaction of antibody-antigen complexes with a Protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  10. Lacour F., Nahon-Merlin E., Michelson M. 1973; Immunological recognition of polynucleotide structure. Current Topics in Microbiology and Immunology 61:1–39
    [Google Scholar]
  11. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  12. Miller J. R., Caliguiri L. A., Tamm I. 1975; Reaction of poliovirus RNAs with antibodies to doublestranded RNA demonstrated by an immunochemical binding assay. Journal of Virology 16:290–297
    [Google Scholar]
  13. Raso V., Schreiber R. 1978; Effects of antibody binding upon thermal transitions of polynucleotides. Biochemistry 17:968–977
    [Google Scholar]
  14. Robertson H. D. 1977; Structure and function of RNA processing signals. In Nucleic Acid-Protein Recognition pp 549–568 Edited by Vogel H. J. New York: Academic Press;
    [Google Scholar]
  15. Roberston H. D., Dickson E., Dunn J. J. 1977; A nucleotide sequence from a ribonuclease III processing site in bacteriophage T7 RNA. Proceedings of the National Academy of Sciences of the United States of America 74:822–826
    [Google Scholar]
  16. Robertson H. D., Dunn J. J. 1975; Ribonucleic acid processing activity of Escherichia coli ribonuclease III. Journal of Biological Chemistry 250:3050–3056
    [Google Scholar]
  17. Scraba D. G., Hostvedt P., Colter J. S. 1970; Physical and chemical studies of mengovirus variants. III. Absorbance-temperature profiles, sedimentation in dextran sulphate gradients and total infectious particle ratios. Canadian Journal of Biochemistry 48:412–417
    [Google Scholar]
  18. Stollar B. D. 1973; Nucleic acid antigens. In The Antigens pp 1–85 Edited by Sela M. New York: Academic Press;
    [Google Scholar]
  19. Stollar B. D. 1975; The specificity and applications of antibodies to helical nucleic acids. CRC Critical Review of Biochemistry 3:45–59
    [Google Scholar]
  20. Wellauer P. K., Dawid I. B. 1973; Secondary structure maps of HeLa ribosomal RNA. Proceedings of the National Academy of Sciences of the United States of America 70:2827–2831
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-3-665
Loading
/content/journal/jgv/10.1099/0022-1317-45-3-665
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error