1887

Abstract

SUMMARY

Pangola stunt virus (PSV) subviral particles (SVPs) were purified from roots and stem bases of (pangola grass); electron microscopy of the preparations showed B-spiked SVPs typical of Fijiviruses. An antiserum of geldiffusion titre 1/1024 was obtained. Serological tests confirmed that both the B spikes and the inner capsid of PSV SVPs were related to those of maize rough dwarf virus (MRDV), though PSV SVPs also carried specific determinants. Isopycnic centrifugation of PSV RNA showed that it is double-stranded, with a G + C content of 41 to 43%. Polyacrylamide gel electrophoresis showed that the RNA consisted of ten segments similar to those of MRDV, though segment eight was present in less than equimolar amounts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-3-659
1979-12-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/3/JV0450030659.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-3-659&mimeType=html&fmt=ahah

References

  1. Boccardo G., Milne R. G. 1975; The maize rough dwarf virion. I. Protein composition and distribution of RNA in different viral fractions. Virology 68:79–85
    [Google Scholar]
  2. Giannotti J., Milne R. G. 1977; Pangola stunt virus in thin sections and in negative stain. Virology 80:347–355
    [Google Scholar]
  3. Ikegami M., Francki R. I. B. 1975; Some properties of RNA from Fiji disease subviral particles. Virology 64:464–470
    [Google Scholar]
  4. Luisoni E., Milne R. G., Boccardo G. 1975; The maize rough dwarf virion. II. Serological analysis. Virology 68:86–96
    [Google Scholar]
  5. Luisoni E., Boccardo G., Milne R. G., Conti M. 1979; Purification, serology and genome of oat sterile dwarf virus. Journal of General Virology 45:651–658
    [Google Scholar]
  6. Matthews R. E. F. 1979; The classification and nomenclature of viruses. Intervirology n:133–135
    [Google Scholar]
  7. Milne R. G. 1977; Pangola stunt virus. CMI/AAB Descriptions of Plant Viruses No. 175, 4. pp
    [Google Scholar]
  8. Milne R. G., Lesemann D.-E. 1978; An immunoelectron microscopic investigation of oat sterile dwarf and related viruses. Virology 90:299–304
    [Google Scholar]
  9. Milne R. G., Lovisolo O. 1977; Maize rough dwarf and related viruses. Advances in Virus Research 21:267–341
    [Google Scholar]
  10. Milne R. G., Luisoni E. 1977; Serological relationships among maize rough dwarf-like viruses. Virology 80:12–20
    [Google Scholar]
  11. Milne R. G., Conti M., Lisa V. 1973; Partial purification, structure and infectivity of complete maize rough dwarf virus particles. Virology 53:130–141
    [Google Scholar]
  12. Milne R. G., Luisoni E., Ling K. C. 1979; Preparation and use of an antiserum to rice ragged stunt virus subviral particles. Plant Disease Reporter 63:445–448
    [Google Scholar]
  13. Reddy D. V. R., Black L. M. 1977; Isolation and replication of mutant populations of wound tumor virions lacking certain genome segments. Virology 80:336–346
    [Google Scholar]
  14. Reddy D. V. R., Boccardo G., Outrtdge R., Teakle D. S., Black L. M. 1975; Electrophorecti separation of dsRN A genome segments from Fiji disease and maize rough dwarf viruses. Virology 63:287–291
    [Google Scholar]
  15. Rubinstein R., Harley E. H. 1978; Reproducible alteration of CPV double-stranded RNA genome patterns on laboratory passage. Virology 84:195–198
    [Google Scholar]
  16. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology 4:430–443
    [Google Scholar]
  17. Shatkin A. J. 1965; Inactivity of purified reovirus RNA as a template for E. coli polymerases in vitro. Proceedings of the National Academy of Sciences of the United States of America 54:1721–1728
    [Google Scholar]
  18. Szybalski W. 1968; Use of cesium sulfate for equilibrium density gradient centrifugation. Methods in Enzymology 12 B:330–360
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-3-659
Loading
/content/journal/jgv/10.1099/0022-1317-45-3-659
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error