1887

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Volume 45, Issue 3

Purification, Serology and Nucleic Acid of Oat Sterile Dwarf Virus Subviral Particles Free

Abstract

SUMMARY

Oat sterile dwarf virus (OSDV) subviral particles (SVPs) were purified from roots and stem bases of . Electron microscopy of the preparations showed B-spiked SVPs typical of Fijiviruses (Fiji disease and maize rough dwarf-like viruses). Two sera with titres in gel-diffusion tests of 1/512 and 1/2048 were prepared against the SVPs. The sera did not react with the SVPs of maize rough dwarf and pangola stunt viruses when tested by gel-diffusion and immuno-electron microscopy. The first serum did not react with virus dsRNA or poly (1).poly(C) and the second serum had a titre of 1/8 with each of these. Poly-acrylamide gel electrophoresis of the nucleic acid from purified OSDV SVPs revealed ten dsRNA segments though only nine of these were resolved as separate bands in dsRNA extracted directly from infected plants. The genome pattern was broadly similar to but distinct from those of the known Fijiviruses. The RNA of the serologically related blue dwarf virus (ABDV), extracted directly from plants, appeared identical to that extracted directly from OSDV-infected plants. Both OSDV and ABDV were transmitted by the planthopper vector despite, in the case of OSDV, having been maintained in vegetatively propagated plants for 5 years.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1979
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1979-12-01
2024-04-23
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References

  1. Boccardo O., Milne R. O. 1975; The maize rough dwarf virion. I. Protein composition and distribution of RNA in different viral fractions. Virology 68:79–85
     [Google Scholar]
  2. Boccardi G., Milne R. O., Luisoni E. 1979; Purification, serology and genome of pangola stunt virus subviral particles. Journal of General Virology 45:659–664
     [Google Scholar]
  3. Catherall P. L. 1970; Oat sterile dwarf virus. Plant Pathology 19:75–78
     [Google Scholar]
  4. Diaz-Ruiz J. R., Kaper J. M. 1977; Cucumber mosiac virus-associated RNA 5. III. Little nucleotide sequence homology between CARNA 5 and helper RNA. Virology 80:204–213
     [Google Scholar]
  5. Ikegami M., Francki R. I. B. 1975; Some properties of RNA from Fiji disease virus subviral particles. Virology 64:464–470
     [Google Scholar]
  6. Matthews R. E. F. 1979; The classification and nomenclature of viruses. Intervirology ii:133–135
     [Google Scholar]
  7. Milne R. G. 1977; Pangola stunt virus. CMI/AAB Descriptions of Plant Viruses No. 175, 4 pp
    [Google Scholar]
  8. Milne R. G., Lesemann D.-E. 1978; An immunoelectron microscopic investigation of oat sterile dwarf and related viruses. Virology 90:299–304
     [Google Scholar]
  9. Milne R. G., Lovisolo O. 1977; Maize rough dwarf and related viruses. Advances in Virus Research 21:267–341
     [Google Scholar]
  10. Milne R. G., Luisoni E. 1977; Serological relationships among maize rough dwarf-like viruses. Virology 80:12–20
     [Google Scholar]
  11. Milne R. G., Conti M., Lisa V. 1973; Partial purification, structure and infectivity of complete maize rough dwarf virus particles. Virology 53:130–141
     [Google Scholar]
  12. Milne R. G., Lindsten K., Conti M. F. 1975; Electron microscopy of the particles of cereal tillering disease virus and oat sterile dwarf virus. Annals of Applied Biology 79:371–373
     [Google Scholar]
  13. Plumb R. T., Misari S. 1974 Rothamsted Annual Report for 1973, Part 1 p 37
     [Google Scholar]
  14. Reddy D. V. R., Black L. M. 1977; Isolation and replication of mutant populations of wound tumor virions lacking certain genome segments. Virology 80:336–346
     [Google Scholar]
  15. Reddy D. V. R., Boccardo G., Outridge R., Teakle D. S., Black L. M. 1975a; Electrophoretic separation of dsRNA genome segments from Fiji disease and maize rough dwarf viruses. Virology 63:287–291
     [Google Scholar]
  16. Reddy D. V. R., Shikata E., Boccardo G., Black L. M. 1975b; . Coelectrophoresis of double-stranded RNA from maize rough dwarf and rice black-streaked dwarf viruses. Virology 67:279–282
     [Google Scholar]
  17. Reddy D. V. R., Grylls N. E., Black L. M. 1976; Electrophoretic separation of dsRNA genome segments from maize wallaby ear virus and its relationship to other phytoreoviruses. Virology 73:36–42
     [Google Scholar]
  18. Redolfi P., Boccardo G. 1974; Fractionation of the double-stranded RNA of maize rough dwarf virus subviral particles. Virology 59:319–322
     [Google Scholar]
  19. Rubinstein R., Harley E. H. 1978; Reproducible alteration of CPV double-stranded RNA genome patterns on laboratory passage. Virology 84:195–198
     [Google Scholar]
  20. Unger J. T., Reichmann M. E. 1973; RNA synthesis in temperature-sensitive mutants of vesicular stomatitis virus. Journal of Virology 12:570–578
     [Google Scholar]
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Purification, Serology and Nucleic Acid of Oat Sterile Dwarf Virus Subviral Particles
J. Gen. Virol. 45, 651 (1979); https://doi.org/10.1099/0022-1317-45-3-651
/content/journal/jgv/10.1099/0022-1317-45-3-651
/content/journal/jgv/10.1099/0022-1317-45-3-651
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