1887

Abstract

SUMMARY

Structural and virus-induced infected cell polypeptides of several strains of influenza B virus were examined by high resolution polyacrylamide gel electrophoresis and shown to be directly analogous to those of the influenza A viruses. Eight structural polypeptides, P, P, P, HA, HA, NA, NP and M were observed in purified virus and at least two additional polypeptides, HA and NS could be detected in infected MDCK cells. The three P proteins plus NP were shown to be associated with RNA-dependent RNA polymerase activity and HA, HA, HA and NA were shown to be glycosylated. Like the influenza A viruses, migrational differences of some of the infected cell polypeptides could be observed between different B strains. Investigation of a time course of virus replication failed to show any temporal control of protein synthesis in the infected cell.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-3-611
1979-12-01
2022-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/3/JV0450030611.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-3-611&mimeType=html&fmt=ahah

References

  1. Almond J. W., Mcgeoch D., Barry R. D. 1977; Method for assigning temperature sensitive mutations of influenza viruses to individual segments of the genome. Virology 81:62–73
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Borland R., Mahy B. W. J. 1968; DNA dependent RNA polymerase activity in cells infected with influenza virus. Journal of Virology 2:33–39
    [Google Scholar]
  4. Chakraverty P. 1971; Antigenic relationships between influenza B viruses. Bulletin of the World Health Organization 45:755–766
    [Google Scholar]
  5. Chakraverty P. 1972; Antigenic relationships between the neuraminidase of influenza B virus. Bulletin of the World Health Organization 46:473–476
    [Google Scholar]
  6. Choppin P. W., Lazarowitz S. G., Goldberg A. R. 1975; Studies on proteolytic cleavage and glycosylation of the haemagglutinin of influenza A and B viruses. In Negative Strand Viruses vol 1 pp 105–119 Edited by Mahy B. W. J. Barry R. D. London, New York and San Francisco: Academic Press;
    [Google Scholar]
  7. Desselberger U., Palese P. 1978; Molecular weights of RNA segments of influenza A and B viruses. Virology 88:394–399
    [Google Scholar]
  8. Francis T., Quilligan J. J., Minuse E. 1950; Identification of another epidemic respiratory disease. Science 112:495–497
    [Google Scholar]
  9. Hay A. J., Lomniczi B., Bellamy A. R., Skehel J. J. 1977; Transcription of the influenza virus genome. Virology 83:337–355
    [Google Scholar]
  10. Hoyle L., Horne R. W., Waterson A. P. 1961; The structure and composition of myxoviruses. II. Components released from the influenza virus particle by ether. Virology 13:448–459
    [Google Scholar]
  11. Inglis S. C., Almond J. W. 1979; An influenza virus gene encoding two different polypeptides. Proceedings of the Royal Society (in the press)
    [Google Scholar]
  12. Inglis S. C., Mahy B. W. J. 1979; Polypeptides specified by the influenza virus genome. 3. Control of synthesis in infected cells. Virology 95:154–164
    [Google Scholar]
  13. Inglis S. C., Carroll A. R., Lamb R. A., Mahy B. W. J. 1976; Polypeptides specified by the influenza virus genome, I. Evidence for eight distinct gene products specified by fowl plague virus. Virology 74:489–503
    [Google Scholar]
  14. Inglis S. C., Mcgeoch D., Mahy B. W. J. 1977; Polypeptides specified by the influenza virus genome. II. Assignment of protein coding functions to individual genome segments by in-vitro translation. Virology 78:522–536
    [Google Scholar]
  15. Kendal A. P. 1975; A comparison of ‘Influenza C’ with prototype myxoviruses: receptor-destroying activity (neuraminidase) and structural polypeptides. Virology 65:87–99
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  17. Lamb R. A., Choppin P. W. 1976; Synthesis of influenza virus proteins in infected cells: translation of viral polypeptides, including three P polypeptides from RNA produced by primary transcription. Virology 74:504–519
    [Google Scholar]
  18. Lamb R. A., Choppin P. W. 1978; Effect of the host cell on early and late synthesis of influenza virus polypeptides. In Negative Strand Viruses and the Host Cell pp 229–237 Edited by Mahy B. W. J., Barry R. D. London, New York and San Francisco: Academic Press;
    [Google Scholar]
  19. Lamb R. A., Etkind P. R., Choppin P. W. 1978; Evidence for a ninth influenza viral polypeptide. Virology 91:60–78
    [Google Scholar]
  20. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  21. Maassab H. F. 1970; Development of variants of influenza virus. In The Biology of Large RNA Viruses pp 542–566 Edited by Mahy B. W. J., Barry R. D. London and New York: Academic Press;
    [Google Scholar]
  22. Nuss D. L., Koch G. 1976; Differential inhibition of vesicular stomatitis virus polypeptide synthesis by hypertonic initiation block. Journal of Virology 17:283–286
    [Google Scholar]
  23. Nuss D. L., Oppermann H., Koch G. 1975; Selective blockage of initiation of host protein synthesis in RNA virus infected cells. Proceedings of the National Academy of Sciences of the United States of American1258–1262
    [Google Scholar]
  24. Oxford J. S. 1973; Polypeptide composition on influenza B viruses and enzymes associated with the purified virus particle. Journal of Virology 12:827–835
    [Google Scholar]
  25. Plotch S. J., Krug R. M. 1977; Influenza virion transcriptase: synthesis in vitro of large polyadenylic acid containing complementary RNA. Journal of Virology 21:24–34
    [Google Scholar]
  26. Racaniello V. R., Palese P. 1978; The genes of influenza B virus: analysis of influenza B virus strains. In Negative Strand Viruses and the Host Cell pp 27–36 Edited by Mahy B. W. J., Barry R. D. London, New York and San Francisco: Academic Press;
    [Google Scholar]
  27. Racaniello V. R., Palese P. 1979; Influenza B virus genome: assignment of viral polypeptides to RNA segments. Journal of Virology 29:361–373
    [Google Scholar]
  28. Riley H. D. Jun 1972; Reye’s syndrome. Journal of Infectious Diseases 125:77–81
    [Google Scholar]
  29. Ritchey M. B., Palese P., Kilbourne E. D. 1976a; RNAs of influenza A, B, and C viruses. Journal of Virology 18:738–744
    [Google Scholar]
  30. Ritchey M. B., Palese P., Schulman J. L. 1976b; Mapping of the influenza virus genome. III. Identification of genes coding for nucleoprotein, membrane protein and non-structural protein. Journal of Virology 20:307–313
    [Google Scholar]
  31. Ritchey M. B., Palese P., Schulman J. L. 1977; Differences in protein patterns of influenza A viruses. Virology 76:122–128
    [Google Scholar]
  32. Rochovansky O. M. 1976; RNA synthesis by ribonucleoprotein-polymerase complexes isolated from influenza virus. Virology 73:327–338
    [Google Scholar]
  33. Rohde W., Harms E., Scholtissek C. 1977; Biochemical studies on influenza viruses. 1. Comparative analysis of equine 2 virus and virus N genes and gene products. Virology 79:393–404
    [Google Scholar]
  34. Saborio J. L., Pong S.-S., Koch G. 1974; Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. Journal of Molecular Biology 85:195–211
    [Google Scholar]
  35. Schild G. C., Pereira M. S., Chakraverty P., Coleman M. T., Dowdle W. R., Chang W. K. F. 1973; Anti gen ic variants of influenza B virus. British Medical Journal 4:127–131
    [Google Scholar]
  36. Skehel J. J. 1972; Polypeptide synthesis in influenza virus infected cells. Virology 49:23–36
    [Google Scholar]
  37. Skehel J. J. 1973; Early polypeptide synthesis in influenza virus infected cells. Virology 56:394–399
    [Google Scholar]
  38. Skehel J. J., Hay A. J. 1978; Nucelotide sequences at the 5′ termini of influenza virus RNAs and their transcripts. Nucleic Acids Research 5:1207–1219
    [Google Scholar]
  39. Tobita K., Kilbourne E. D. 1975; Structural polypeptides of antigenically distinct strains of influenza B virus. Archives of Virology 47:367–374
    [Google Scholar]
  40. Ueda M., Tobita K., Sugiura A., Enomoto C. 1978; Identification of haemagglutinin and neuraminidase genes of influenza B virus. Journal of Virology 25:685–686
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-3-611
Loading
/content/journal/jgv/10.1099/0022-1317-45-3-611
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error