1887

Abstract

SUMMARY

The structural polypeptides of accepted species and recently isolated members of the genus have been examined by SDS-polyacrylamide gel electrophoresis. The viruses shared many polypeptides but some differences were found. The viruses could be divided into a vaccinia group (including buffalopox, ‘Lenny’ and MK-10), an ectromelia group (including elephant virus and Moscow virus), cowpox, camelpox and monkeypox. Minor differences were found in the polypeptides of monkeypox virus strains from human and monkey outbreaks. Controlled degradation of virions showed that the polypeptides which enabled the viruses to be differentiated were located in the surface and sub-surface layers. The cores of the viruses all gave the same complex polypeptide pattern.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-3-537
1979-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/3/JV0450030537.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-3-537&mimeType=html&fmt=ahah

References

  1. Arita M., Tagaya I. 1977; Structural polypeptides of several strains of orthopoxvirus. Microbiology and Immunology 21:343–346
    [Google Scholar]
  2. Baxby D. 1972; A comparison of the antigens present on the surface of virus released artificially from chick cells infected with vaccinia virus, and cowpox virus and its white pock mutant. Journal of Hygiene 70:353–365
    [Google Scholar]
  3. Baxby D., Ghaboosi B. 1977; Laboratory characteristics of poxviruses isolated from captive elephants in Germany. Journal of General Virology 37:407–414
    [Google Scholar]
  4. Bourke A. T. C., Dumbell K. R. 1972; An unusual poxvirus from Nigeria. Bulletin of the World Health Organization 46:621–623
    [Google Scholar]
  5. Easterbrook K. B. 1966; Controlled degradation of vaccinia virions in vitro: an electron microscopic study. Journal of Ultrastructure Research 14:484–496
    [Google Scholar]
  6. Esposito J. J., Obueski J. F., Nakano J. H. 1977; The virion and soluble antigen proteins of variola, monkeypox and vaccinia viruses. Journal of Medical Virology I:95–110
    [Google Scholar]
  7. Esposito J. J., Obijeski J. F., Nakano J. H. 1978; Orthopoxvirus DNA: strain differentiation by electrophoresis of restriction endonuclease fragmented virion DNA. Virology 89:53–66
    [Google Scholar]
  8. Fairbanks G., Steck T. L., Wallach D. F. M. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2617
    [Google Scholar]
  9. Holowczak J. A., Joklik W. K. 1967; Studies on the proteins of vaccinia virus, I. Structural proteins of the virion and core. Virology 33:717–725
    [Google Scholar]
  10. Katz E., Margalith E. 1973; Location of vaccinia virus structural polypeptides on the surface of the virus particle. Journal of General Virology 18:381–384
    [Google Scholar]
  11. Marennikova S. S., Ladnyj I. D., Ogorodnikova Z. I., Shelukhina E. M., Maltseva N. N. 1978; Identification and study of a poxvirus isolated from wild rodents in Turkmenia. Archives of Virology 56:7–14
    [Google Scholar]
  12. Pennington T. H. 1973; Vaccinia virus morphogenesis: a comparison of virus-induced antigens and polypeptides. Journal of General Virology 19:65–79
    [Google Scholar]
  13. Rondle C. J. M., Sayeed K. A. R. 1972; Studies on monkeypox virus. Bulletin of the World Health Organization 46:577–583
    [Google Scholar]
  14. Sarov I., Joklik W. K. 1972; Studies on the nature and location of the capsid polypeptides of vaccinia virions. Virology 50:579–592
    [Google Scholar]
  15. Shelukhina E. M., Maltseva M. N., Shenkman L. S., Marennikova S. S. 1975; Properties of two isolates (MK-7-73 and MK-10-73) from wild monkeys. British Veterinary Journal 131:746–748
    [Google Scholar]
  16. Shida H., Tanake K., Matsumoto S. 1977; Mechanism of virus occlusion into A-type inclusions during poxvirus infection. Virology 76:217–233
    [Google Scholar]
  17. Stern W., Dales S. 1976; Biogenesis of vaccinia: isolation and characterization of a surface component that elicits antibody suppressing infectivity and cell-cell fusion. Virology 75:232–241
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-3-537
Loading
/content/journal/jgv/10.1099/0022-1317-45-3-537
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error