1887

Abstract

SUMMARY

The capsid of bacteriophage SP3 is assembled via a prohead intermediate which subsequently encapsulates DNA and attaches a tail. The prohead contains a ring-like core structure. The spokes which extend from the core to the inner prohead surface are thought to form a scaffold for the polymerization of the prohead. Ninety percent of the proheads are assembled prior to the onset of DNA encapsulation. The first mature phage particles are observed at 45 min after infection; titres of intracellular phage demonstrate their infectivity. The core is visible in phage ghosts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-2-515
1979-11-01
2024-11-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/2/JV0450020515.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-2-515&mimeType=html&fmt=ahah

References

  1. Botstein D., Waddel C., King J. 1973; Mechanism of head assembly and DNA encapsulation in Salmonella phage P22. I. Genes, proteins, structures, and DNA maturation. Journal of Molecular Biology 80:669–695
    [Google Scholar]
  2. Casjens S., King J. 1974; P22 morphogenesis. I. Catalytic scaffolding protein in capsid assembly. Journal of Supramoleeular Structure 2:202–224
    [Google Scholar]
  3. Eiserling F. A., Romig W. 1962; Studies of Bacillus subtilis bacteriophages. Structural characterization by electron microscopy. Journal of Ultrastructure Research 6:540–546
    [Google Scholar]
  4. Hagen E. W., Reilly B. F., Tosi M. E., Andfrson D. L. 1976; Analysis of gene function of bacteriophage ϕ 29 of Bacillus subtilis: identification of cistrons essential for viral assembly. Journal of Virology 19:501–517
    [Google Scholar]
  5. Hohn B., Hohn T. 1974; Activity of empty, headlike particles for packaging of DNA of phage λ in vitro . Proceedings of the National Academy of Sciences of the United States of America 71:2372–2376
    [Google Scholar]
  6. Kellenberger F., Eiserling F. A., Boy De La Tour E. 1968; Studies on the morphogenesis of the head of phage T–even. III. The cores of head–related structures. Journal of Ultrastructure Research 21:335–360
    [Google Scholar]
  7. King J., Lenk E., Botstein D. 1973; Mechanism of bead assembly and DNA encapsulation in Salmonella phage P22. II. Morphogenetic pathway. Journal of Molecular Biology 80:697–731
    [Google Scholar]
  8. Laemmli V. K., Favre M. 1973; Maturation of the head of bacteriophage T4.1. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  9. Lenk E., Casjens S., Weeks J., King J. 1975; Intracellular visualization of precursor capsids in phage P22 mutant infected cells. Virology 68:182–199
    [Google Scholar]
  10. Luftig R., Wood W., Okinaka R. 1971; Bacteriophage T4 head morphogenesis: on the nature of gene 49–defective heads and their role as intermediates. Journal of Molecular Biology 57:555–573
    [Google Scholar]
  11. Matsuo–Kato H., Fujisawa H. 1975; Studies on bacteriophage T3. III. Characterization of capsids as intermediates in head assembly. Virology 63:105–114
    [Google Scholar]
  12. Murialdo H., Becker A. 1978; Head morphogenesis of complex double–stranded deoxyribonucleic–acid bacteriophages. Microbiological Reviews 42:529–576
    [Google Scholar]
  13. Nelson R. A., Reilly B. E., Anderson D. L. 1976; Morphogenesis of bacteriophage ϕ 29 of B. subtilis: preliminary isolation and characterization of intermediate particles of the assembly pathway. Journal of Virology 19:518–532
    [Google Scholar]
  14. Nishihara M., Romig W. R. 1964; Temperature–sensitive mutants of Bacillus subtilis bacteriophage SP3. I. Isolation and characterization. Journal of Bacteriology 88:1220–1229
    [Google Scholar]
  15. Pruss G., Goldstein R., Calendar R. 1974; In vitro packaging of satellite P4 DNA. Proceedings of the National Academy of Sciences of the United States of America 71:2367–2371
    [Google Scholar]
  16. Roeder G., Sadowski R. 1977; Bacteriophage T7 morphogenesis: phage related particles. Virology 76:263–285
    [Google Scholar]
  17. Serwer P. 1978; A technique for observing extended DNA in negatively stained specimens: observation of bacteriophage T7 capsid–DN A complexes. Journal of Ultrastructure Research 65:112–118
    [Google Scholar]
  18. Simon L. D. 1972; Infection of E. coli by T2 and T4 bacteriophage as seen in the electron microscope: T4 head morphogenesis. Proceedings of the National Academy of Sciences of the United States of America 69:907–911
    [Google Scholar]
  19. Venable J. H., Coggleshall R. 1965; A simplified lead citrate stain for use in electron microscopy. Journal of Cell Biology 25:407–408
    [Google Scholar]
  20. Zachary A., Simon L. D., Litwin S. 1976; Lambda head morphogenesis as seen in the electron micro–scope. Virology 72429–442
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-45-2-515
Loading
/content/journal/jgv/10.1099/0022-1317-45-2-515
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error