The RNA-dependent RNA polymerase induced in BHK 21 cells by infection with foot-and-mouth disease virus has been isolated from the replication complex. It contains a major, virus-coded protein with mol. wt. 56000 which appears from serological studies and tryptic peptide mapping to be the same as the virus infection associated (VIA) antigen and the protein P56 found in cells infected with the virus. Other virus coded proteins and a host cell protein were present in the partially purified replication complex but were removed by digestion with ribonuclease T1, leaving only the major virus coded protein. The tryptic peptide maps of the VIA antigen of the seven serotypes of the virus were similar, suggesting a high level of conservation in that region of the genome coding for the RNA polymerase of each type.
BlackD. N.,
BrownF.1969; Effect of actinomycin D and guanidine on the formation of a ribonucleic acid polymerase induced by foot–and–mouth disease virus and on the replication of virus and viral ribonucleic acid. Biochemical Journal 112:317–323
CooperP.,
WentworthB.,
MccahonD.1970; Guanidine inhibition of poliovirus. A dependence of viral RNA synthesis on the configuration of structural protein. Virology 40:486–493
CowanK. M.,
TrautmanR.1967; Immuno–chemical studies of foot–and–mouth disease. 1. Complement fixation reactions with isolated antigenic components. Journal of Immunology 99:729–736
DmitrievaT. M.,
ShcheglovaM. V.,
AgolV. I.1979; Inhibition of activity of encephalomyocarditis virus induced RNA polymerase by antibodies against cellular components. Virology 92:271–277
DoelT. R.,
SangarD. V.,
RowlandsD. J.,
BrownF.1978; A re–appraisal of the biochemical map of foot–and–mouth disease virus RNA. Journal of General Virology 41:395–404
GhendonY.,
BabushkinaL.,
BlagoveshienskayaO.1973; Inhibition of poliovirus RNA synthesis in an in vitro system by antiserum against 14S virus–specific structures. Archiv für die gesamte Virusforschung 40:47–51
GoelzS.,
SteitzJ. A.1977; Escherichia coli ribosomal protein S1 recognises two sites in bacteriophage Qβ RNA. Journal of Biological Chemistry 252:5177–5179
LundquistR. E.,
EhrenfeldE.,
MaizelJ. V.1974; Isolation of a viral polypeptide associated with poliovirus replication complex. Proceedings of the National Academy of Sciences of the United States of America 71:4773–4777
PorterA. G.,
FellnerP.,
BlackD. N.,
RowlandsD. J.,
HarrisT. J. R.,
BrownF.1978; 3′–Terminal nucleotide sequences in the genome RNA of picornaviruses. Nature, London 276:298–301
RobsonK. J. H.,
HarrisT. J. R.,
BrownF.1977; An assessment by competition hybridisation of the sequence homology between RNAs of the seven serotypes of FMDV. Journal of General Virology 37:271–276
SangarD. V.,
RowlandsD. J.,
CavanaghD.,
BrownF.1976; Characterization of the minor polypeptides in the foot–and–mouth disease particle. Journal of General Virology 31:35–46
ShihD. S.,
ShihC. T.,
KewO.,
PallanschM.,
RueckertR.,
KaesbergP.1979; Cell free synthesis and processing of the proteins of poliovirus. Proceedings of the National Academy of Sciences of the United States of America 75:5807–5811
TraubA.,
DiskinB.,
RosenbergH.,
KalmarE.1976; Isolation and properties of the replicase of en– cephalomyocarditis virus. Journal of Virology 18:375–382
WeckerE.,
LederhilgerG.1964; Genomic masking produced by double infection of HeLa cells with heterotypic polioviruses. Proceedings of the National Academy of Sciences of the United States of America 52:705–709