1887

Abstract

SUMMARY

A heterogeneous population of virions is generated by measles virus-infected cells. These particles are partially separable by sucrose density centrifugation into three peaks. Each population is stable and contains infectious particles. The particles of all three populations contain at least six polypeptide species that differ between particle populations only in quantity. All three populations contain a 50S RNA species, and the heaviest density peak also contains an additional species of 43S RNA. The difference between these results and previous studies with measles virions will be discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-2-441
1979-11-01
2022-08-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/2/JV0450020441.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-2-441&mimeType=html&fmt=ahah

References

  1. Baker R. F., Gordon I., Stevenson D. 1965; Electron microscope study of hemadsorption in measles virus infection. Virology 27:441–445
    [Google Scholar]
  2. Bratt M. A., Hightower L. E. 1977; Genetics and paragenetic phenomena of paramyxovirus. In Comprehensive Virology vol 9 pp 457–533 Edited by Fraenkel-Conrat H. Wagner R. R. New York and London: Plenum Press;
    [Google Scholar]
  3. Burnstein T. T., Jensen J. H., Waksman B. H. 1964; The development of a neurotropic strain of measles virus in hamsters and mice. Journal of Infectious Diseases 114:265
    [Google Scholar]
  4. Bussell R. H., Waters D. J., Seals M. K., Robinson W. S. 1974; Measles, canine distemper, and respiratory syncytial virions and nucleocapsids. A comparative study of their structure, polypeptide and nucleic acid composition. Medical Microbiology and Immunology 160:105–124
    [Google Scholar]
  5. Chiarini A., Norrby E. 1970; Separation and characterization of products of two measles virus variants. Archiv fur die gesamte Virusforschung 29:204–214
    [Google Scholar]
  6. Granoff A. 1959; Studies on mixed infection with Newcastle disease virus II. The occurrence of Newcastle disease virus heterozygotes and study of phenotypic mixing involving serotypes and thermal stability. Virology 9:649–670
    [Google Scholar]
  7. Graves M. C., Silver S. M., Choppin P. W. 1978; Measles virus polypeptide synthesis in infected cells. Virology 86:254–263
    [Google Scholar]
  8. Hall W. W., Martin S. J. 1973; Purification and characterization of measles virus. Journal of General Virology 19:175–188
    [Google Scholar]
  9. Hall W. W., Martin S. J. 1974a; Structure and function relationships of the envelope of measles virus. Medical Microbiology and Immunology 160:143–154
    [Google Scholar]
  10. Hall W. W., Martin S. J. 1974b; Defective interfering particles produced during the replication of measles virus. Medical Microbiology and Immunology 160:155–164
    [Google Scholar]
  11. Hall W. W., Ter Meulen V. 1977; Polyadenylic acid [Poly(A)] sequences associated with measles virus intracellular ribonucleic acid (RNA) species. Journal of General Virology 35:497–510
    [Google Scholar]
  12. Hardwicke J. M., Bussell R. H. 1978; Glycoproteins of measles virus under reducing and non–reducing conditions. Journal of Virology 25:687–692
    [Google Scholar]
  13. Kiley M. P., Gray R. H., Payne F. E. 1974; Replication of measles virus: distinct species of short nucleocapsids in cytoplasmic extracts of infected cells. Journal of Virology 13:721–728
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of the bacteriophage T–4. Nature, London 227:680–685
    [Google Scholar]
  15. Ledeen R. W., Miller C. A., Haley J. E., Raine C. S. 1976; Lipids and slow viruses: comparison of measles and SSPE virions. In Current Trends in Sphingolipidosis and Allied Disorders pp 567–584 Edited by Volk B. Schneck L. New York: Plenum;
    [Google Scholar]
  16. Levine E. M. 1972; Mycoplasma contamination of animal cell cultures: a simple, rapid assay for detection of mycoplasma. Experimental Cell Research 74:99–109
    [Google Scholar]
  17. Maizel J. V. 1969; Acrylamide gel electrophoresis of proteins and nucleic acids. In Fundamental Techniques in Virology pp 334–362 Edited by Habel K. Salzman N. P. New York: Academic Press;
    [Google Scholar]
  18. Mountcastle W. E., Choppin P. W. 1977; A comparison of four measles virus strains. Virology 78:463–474
    [Google Scholar]
  19. Penman S. 1969; Preparations of purified nuclei and nucleoli from mammalian cells. In Fundamental Techniques in Virology pp 35–48 Edited by Habel K. Salzman N. P. New York: Academic Press;
    [Google Scholar]
  20. Rager–Zisman B., Merigan T. 1973; A useful quantitative semimicromethod for viral plaque assay. Proceedings of the Society for Experimental Biology and Medicine 142:1174–1179
    [Google Scholar]
  21. Raine C. S., Feldman L. A., Sheppard R. D., Bornstein M. B. 1969; Ultrastructure of measles virus in cultures of hamster cerebellum. Journal of Virology 4:169–187
    [Google Scholar]
  22. Rapp F. 1964; Plaque differentiation and replication of virulent and attenuated strains of measles virus. Journal of Bacteriology 88:1448–1458
    [Google Scholar]
  23. Rapp F., Gordon I., Baker R. F. 1960; Observation of measles virus infection of cultured human cells. I. A study of development and spread of virus antigen by means of immunofluorescence. Journal of Biophysical and Biochemical Cytology 7:43–48
    [Google Scholar]
  24. Roman J. A., Simon E. H. 1976; Morphologic heterogeneity in egg– and monolayer–propagated Newcastle disease virus. Virology 69:287–297
    [Google Scholar]
  25. Schleuderberg A. E. 1971; Measles virus RNA. Biochemical and Biophysical Research Communications 42:1012–1015
    [Google Scholar]
  26. Stewart M. L., Summers D. F., Soiero R., Fields B., Maizel J. V. 1973; Purification of oncornavirus with Concanavalin A. Proceedings of the National Academy of Sciences of the United States of America 70:1308–1312
    [Google Scholar]
  27. Tyrrell D. L. G., Norrby E. 1978; Structural polypeptides of measles virus. Journal of General Virology 39:219–229
    [Google Scholar]
  28. Waters D. J., Bussell R. H. 1973; Polypeptide composition of measles and canine distemper viruses. Virology 55:554–557
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-45-2-441
Loading
/content/journal/jgv/10.1099/0022-1317-45-2-441
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error