1887

Abstract

SUMMARY

Cucumber mosaic virus (CMV) strains S and WT were selected as reference viruses to provide the double-stranded and H-labelled single-stranded RNAs in competition hybridization experiments which determined the degree of nucleotide sequence homology among 18 CMV isolates of different origin. A group of 3 and a group of 14 isolates (S and WT groups, respectively) were found to have extensive, if not total, sequence homology among their members and little, if any, homology between the groups. Only one isolate obtained from Ixora (CMV-Ix) was partially homologous (43%) with the WT group and had no homology with the S group. The finding of two main groups of CMV isolates, based on nucleic acid homology, parallels the divisions on the basis of symptomatology and serological properties proposed previously by other workers.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-45-2-361
1979-11-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/45/2/JV0450020361.html?itemId=/content/journal/jgv/10.1099/0022-1317-45-2-361&mimeType=html&fmt=ahah

References

  1. Bol J. F., Brederode F. T., Janze O. C., Rauh D. K. 1975; Studies on sequence homology between the RNAs of alfalfa mosaic virus. Virology 65:1–15
    [Google Scholar]
  2. Clewley J. P., Bishop D. H. L., Kang C.-Y., Coffin J., Schnitzlein W. M., Reichmann M. E., Shope R. E. 1977; Oligonucleotide fingerprints of RNA species obtained from Rhabdoviruses belonging to the vesicular stomatitis virus subgroup. Journal of Virology 23:152–166
    [Google Scholar]
  3. Darby G., Minson A. C. 1973; The structure of tobacco rattle virus ribonucleic acids: common nucleotide sequences in the RNA species. Journal ofGeneral Virology 21:285–295
    [Google Scholar]
  4. Devergne J. C., Cardin L. 1973; Contribution al’étude du virus de la mosaique du concombre (CMV). Annales de Phytopathologie 5:409–430
    [Google Scholar]
  5. Diaz–Ruiz J. R., Kaper J. M. 1977; Cucumber mosaic virus–associated RNA 5. III. Little nucleotide sequence homology between CARNA 5 and helper RNA. Virology 80:203–213
    [Google Scholar]
  6. Diaz-Ruiz J. R., Kaper J. M. 1978; Isolation of viral double–stranded RNAs using a LiCl fractionation procedure. Preparative Biochemistry 8:1–17
    [Google Scholar]
  7. Fulton J. P. 1950; Studies on strains of cucumber virus 1 from spinach. Phytopathology 40:729–736
    [Google Scholar]
  8. Gonda T. J., Symons R. H. 1978; The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its applications to several strains of cucumoviruses. Virology 88:361–370
    [Google Scholar]
  9. Gould A. R., Symons R. H. 1977; Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA. Nucleic Acids Research 4:3787–3802
    [Google Scholar]
  10. Kaper J. M., Tousignant M. E. 1977; Cucumber mosaic virus–associated RNA 5. I. Role of host plant and helper strain in determining amount of associated RNA 5 with virions. Virology 80:186–195
    [Google Scholar]
  11. Kaper J. M., Tousignant M. E. 1978; Cucumber mosaic virus–associated RNA 5. V. Extensive nucleotide sequence homology among CARNA 5 preparations of different CMV strains. Virology 85:323–327
    [Google Scholar]
  12. Kaper J. M., Tousignant M. E., Lot H. 1976; A low molecular weight replicating RNA associated with a divided genome plant virus. defective or satellite RNA Biochemical and Biophysical Research Communications 72:1237–1243
    [Google Scholar]
  13. Kummert J., Lacroix J. P., Semal J. 1978; Heterology among the RNAs oftymoviruses as revealed by RNA–RNA hybridizations. Virology 89:306–308
    [Google Scholar]
  14. Lot H., Kaper J. M. 1976; Physical and chemical differentiation of three strains of cucumber mosaic virus and peanut stunt virus. Virology 74:209–222
    [Google Scholar]
  15. Lot H., Jonard G., Richards K. E. 1977; Cucumber mosaic virus RNA 5. Partial characterization and evidence for no large sequence homologies with genomic RNAs. Federation of European Biochemical Societies Letters 80:395–400
    [Google Scholar]
  16. Marrou J., Quiot J. B., Marchoux G. 1975; Caracterisation de Douzesouches du VMC par leurs aptitudes pathogénes: tentative de classification. Mededelingen Faculteit Landbouwwetenschappen Rijk – suniversiteit Gent 40:107–122
    [Google Scholar]
  17. Mink G. I., Silbernagel M. J., Saksena K. N. 1969; Host range, purification, and properties of the western strain of peanut stunt virus. Phytopathology 59:1625–1631
    [Google Scholar]
  18. Palomar M. K., Brakke M. K., Jackson A. O. 1977; Base sequence homology in the RNAs of barley stripe mosaic virus. Virology 77:471–480
    [Google Scholar]
  19. Price W. C. 1934; Isolation and study of some yellow strains of cucumber mosaic virus. Phytopathology 24:742–761
    [Google Scholar]
  20. Robson K. J. H., Harris T. J. R., Brown F. 1977; An assessment by competition hybridization of the sequence homology between the RNAs of the seven serotypes of FMDV. Journal of General Virology 37:271–276
    [Google Scholar]
  21. Tolin S. A. 1977; Cucumovirus (cucumber mosaic virus) group. In The Atlas ofInsects and Plant Viruses, Including Mycoplasma Viruses and Viroids pp 303–309 New York: Academic Press;
    [Google Scholar]
  22. Troutman J. L., Fulton R. W. 1958; Resistance in tobacco to cucumber mosaic virus. Virology 6:303–316
    [Google Scholar]
  23. Vandewalle M. J., Siegel A. 1976; A study of nucleotide sequence homology between strains of tobacco mosaic virus. Virology 73:413–418
    [Google Scholar]
  24. Waterworth H. E., Tousignant M. E., Kaper J. M. 1978; A lethal disease of tomato experimentally induced by RNA–5 associated with cucumber mosaic virus isolated from Commelina from El Salvador. Phytopathology 68:561–566
    [Google Scholar]
  25. Wengler G., Wengler G., Filipe A. R. 1977; A study of nucleotide sequence homology between the nucleic acids of different alphaviruses. Virology 78:124–134
    [Google Scholar]
  26. Young N. A. 1973; Polioviruses, coxsackieviruses, and Echoviruses: comparison of the genomes by RNA hybridization. Journal of Virology 11:832–839
    [Google Scholar]
  27. Zaitlin M., Beachy R. N., Breuning G. 1977; Lack of molecular hybridization between RNAs of two strains of TMV: a reconsideration of the criteria for strain relationship. Virology 82:237–241
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-45-2-361
Loading
/content/journal/jgv/10.1099/0022-1317-45-2-361
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error