Two polypeptides (imm-a and imm-b) which are not induced by an immunity mutant T4Dimm2 but by a wild-type strain T4D were identified by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. Their mol. wt. were 77000 and 45000, respectively. These polypeptides exhibited a similar kinetic pattern of synthesis. Within a few minutes p.i. the primary phage established the system that inhibited imm gene expression of superinfecting phage. This was shown by measuring both the phenotypic expression of immunity and the synthesis of imm gene polypeptides. The expression of two other immediate-early genes, namely genes s and 30, and the early gene 33, was not affected by primary infection.
DaegelenP.,
BrodyE.1976; Early bacteriophage T4 transcription. A diffusible product controls rIIA and rIIB RNA synthesis. Journal of Molecular Biology 103:127–142
IshibashiM.,
MaizelJ. V.Jun1974; The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology 57:409–424
JayaramanR.1972; Transcription of bacteriophage T4 DNA by Escherichia coli RNA polymerase in vitro:identification of some immediate-early and delayed-early genes. Journal of Molecular Biology 70:253–263
MarshR. C.,
BreschkinA. M.,
MosigG.1971; Origin and direction of bacteriophage T4 DNA replication. II. A gradient of marker frequencies in partially replicated T4 DNA as assayed by transformation. Journal of Molecular Biology 60:213–233
O’farrellP. Z.,
GoldL. M.1973a; Bacteriophage T4 gene expression. Evidence for two classes of prereplicativecistrons. Journal of Biological Chemistry 248:5502–5511
O’farrellP. Z.,
GoldL. M.1973b; Transcription and translation of prereplicative bacteriophage T4 genes in vitro. Journal of Biological Chemistry 248:5512–5519
SalserW.,
BolleA.,
EpsteinR.1970; Transcription during bacteriophage T4 development: a demonstration that distinct subclasses of the ‘early’ RNA appear at different times and that some are ‘turned off’ at late times. Journal of Molecular Biology 49:271–295
TomizawaJ.,
AnrakuN.1964; Molecular mechanisms of genetic recombination in bacteriophage. I. Effect of KCN on genetic recombination of phage T4
. Journal ofMolecular Biology 8:508–515
ValleeM.,
CornettJ. B.1972; A new gene of bacteriophage T4 determining immunity against super-infecting ghosts and phage in T4-infected Escherichia coli. Virology 48:777–784
YutsudoM.,
OkamotoK.1973; Immediate-early expression of the gene causing superinfection breakdown in bacteriophage T4B. Journal of Virology 12:1628–1630